Skibo G G, Nikonenko I R, Rusakov D A, Berezovskaia O L, Leterier Zh -F, Lepekhin E A
Department of Cytology, Bogomoletz Institute of Physiology, Kiev, Ukraine.
Morfologiia. 1999;115(3):41-8.
The spinal cord and hippocampal primary cultures were incubated with three neurotoxins (separately) known to impair the main components of the cytoplasmic cytoskeleton: 1) colchicine blocking the repolymerization of microtubules, 2) cytochalasin preventing elongation of actin filaments, and 3) beta, beta'-iminodipropionitrile (IDPN), causing disorganisation of neurofilaments. The distribution of surface membrane molecules on the surface of the neurons was evaluated in the ultrastructural study after treatment with the neurotoxins on the 5th, 12th, and 15th days in vitro (DIV). On the 12 DIV, the density of immunogold labelled neural cell adhesion molecules (NCAM) on IDPN-treated hippocampal neurons increased 1.45 times comparing to the controls. On the 5 DIV, the density of WGA (wheat germ agglutinin)-binding membrane glycoproteins increased 2.09 times on colchicine-treated neurons, and 3.98 times on cytochalasin-treated ones, whereas on the 12 DIV, the increase was 3.28 and 2.72 times, respectively, as compared to the control cultures of the same age. These data provide insights into the mechanisms of neurodegenerative changes in the nerve cells and into the relationship between the cytoskeletal elements and the surface molecules on the neuronal plasmatic membrane.
1）秋水仙碱，它会阻断微管的再聚合；2）细胞松弛素，它会阻止肌动蛋白丝的伸长；3）β,β'-亚氨基二丙腈（IDPN），它会导致神经丝紊乱。在体外培养的第5天、第12天和第15天用神经毒素处理后，通过超微结构研究评估神经元表面膜分子的分布。在体外培养第12天时，与对照组相比，经IDPN处理的海马神经元上免疫金标记的神经细胞黏附分子（NCAM）密度增加了1.45倍。在体外培养第5天时，经秋水仙碱处理的神经元上麦胚凝集素（WGA）结合膜糖蛋白的密度增加了2.09倍，经细胞松弛素处理的神经元上增加了3.98倍，而在体外培养第12天时，与相同年龄的对照培养物相比，增加倍数分别为3.28倍和2.72倍。这些数据为神经细胞神经退行性变化的机制以及细胞骨架成分与神经元质膜表面分子之间的关系提供了见解。
