增殖性视网膜疾病玻璃体切除标本的免疫组织化学研究
[Immunohistochemical study on vitrectomy specimens of proliferative retinal diseases].
作者信息
Cao J, Wu L, Zhang H
机构信息
Department of Ophthalmology, Third Hospital, Beijing Medical University.
出版信息
Zhonghua Yan Ke Za Zhi. 1997 Jul;33(4):264-7.
OBJECTIVE
To evaluate the characteristics of cellular proliferation in different retinal diseases.
METHODS
Three specific antibodies [to cytokeratin (CK), glial fibrillary acidic protein (GFAP) and actin, respectively] were used. Vitrectomy specimens from 28 cases with different proliferative retinal diseases were studied immunohistochemically.
RESULTS
In the vitreous with proliferative vetreoretinopathy (PVR), most of the proliferative cells were derived from glial and retinal pigment epithelial (RPE) cells. In the vitreous of proliferative diabetic retinopathy (PDR), RPE cells might enhance the contraction of proliferative membranes. Increase in actin in firovascular membranes with PDR may play a positive role on the pericytes dropping out from microvessels.
CONCLUSION
The cell proliferation in the vitreous associated with the increase of levels of growth factors in pathological vitreous, possibly, is one of the mechanisms for the deterioration of proliferative retinal diseases.
目的
评估不同视网膜疾病中细胞增殖的特征。
方法
使用三种特异性抗体（分别针对细胞角蛋白（CK）、胶质纤维酸性蛋白（GFAP）和肌动蛋白）。对28例不同增殖性视网膜疾病患者的玻璃体切除标本进行免疫组织化学研究。
结果
在增生性玻璃体视网膜病变（PVR）的玻璃体中，大多数增殖细胞来源于神经胶质细胞和视网膜色素上皮（RPE）细胞。在增殖性糖尿病视网膜病变（PDR）的玻璃体中，RPE细胞可能增强增殖膜的收缩。PDR的纤维血管膜中肌动蛋白增加可能对周细胞从微血管脱落起积极作用。
结论
玻璃体中的细胞增殖可能与病理性玻璃体中生长因子水平升高有关，是增殖性视网膜疾病恶化的机制之一。
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