增殖性糖尿病视网膜病变和增殖性玻璃体视网膜病变中的趋化因子

Chemokines in proliferative diabetic retinopathy and proliferative vitreoretinopathy.

作者信息

Abu El-Asrar Ahmed M, Struyf Sofie, Kangave Dustan, Geboes Karel, Van Damme Jo

机构信息

Department of Ophthalmology, College of Medicine, King Saud University, King Abdulaziz University Hospital, Airport Road, PO Box 245, Riyadh 11411, Saudi Arabia.

出版信息

Eur Cytokine Netw. 2006 Sep;17(3):155-65.

PMID:17194635

Abstract

PURPOSE

To determine levels of the chemokines CCL1/I-309, CCL2/MCP-1, CCL3/MIP-1alpha, CCL4/MIP-1beta, CCL7/MCP-3, CCL8/MCP-2, CXCL5/ENA-78, CXCL6/GCP-2, CXCL10/IP-10, and CXCL11/I-TAC in the vitreous humor and serum, from patients with proliferative diabetic retinopathy (PDR), proliferative vitreoretinopathy (PVR), and rhegmatogenous retinal detachment with no PVR (RD), and to investigate the expression of MCP-1, CXCL12/SDF-1, and the chemokine receptor CXCR3 in epiretinal membranes.

METHODS

Paired vitreous humor and serum samples were obtained from patients undergoing vitrectomy for the treatment of RD (57 specimens), PVR (32 specimens), and PDR (88 specimens). The levels of chemokines were measured by enzyme-linked immunosorbent assays. Eighteen PDR and 5 PVR membranes were studied by immunohistochemical techniques.

RESULTS

Of all the chemokines studied, only MCP-1 and IP-10 were detected in vitreous humor samples. MCP-1 levels in vitreous humor samples were significantly higher than in serum samples (p < 0.001). MCP-1 levels were significantly higher in vitreous humor samples from patients with PVR and PDR compared with RD (p = 0.0002). MCP-1 levels in vitreous humor samples from patients with active PDR were significantly higher than in inactive PDR cases (p = 0.0224). IP-10 levels in vitreous humor samples were significantly higher than in serum samples (p = 0.0035). IP-10 levels were significantly higher in vitreous humor samples from patients with PVR and PDR compared with RD (p = 0.0083). The incidence of IP-10 detection in vitreous humor samples was significantly higher in active PDR cases compared with inactive cases (p = 0.0214). There was a significant association between the incidence of IP-10 detection and increased levels of MCP-1 in vitreous humor samples from all patients, and patients with RD and PDR (p < 0.001 for all comparisons). MCP-1, and SDF-1 were localized in myofibroblasts in PVR and PDR membranes and in vascular endothelial cells in PDR membranes. CXCR3 was expressed by vascular endothelial cells in PDR membranes.

CONCLUSION

MCP-1, IP-10 and SDF-1 may participate in pathogenesis of PVR and PDR. Myofibroblasts and vascular endothelial cells are the major cell types expressing MCP-1, SDF-1, and CXCR3 in epiretinal membranes.

摘要

目的

测定增殖性糖尿病视网膜病变（PDR）、增殖性玻璃体视网膜病变（PVR）以及无PVR的孔源性视网膜脱离（RD）患者玻璃体液和血清中趋化因子CCL1/I - 309、CCL2/MCP - 1、CCL3/MIP - 1α、CCL4/MIP - 1β、CCL7/MCP - 3、CCL8/MCP - 2、CXCL5/ENA - 78、CXCL6/GCP - 2、CXCL10/IP - 10和CXCL11/I - TAC的水平，并研究视网膜前膜中MCP - 1、CXCL12/SDF - 1和趋化因子受体CXCR3的表达。

方法

从接受玻璃体切除术治疗RD（57份样本）、PVR（32份样本）和PDR（88份样本）的患者中获取配对的玻璃体液和血清样本。通过酶联免疫吸附测定法测量趋化因子水平。采用免疫组织化学技术研究18份PDR和5份PVR膜。

结果

在所有研究的趋化因子中，仅在玻璃体液样本中检测到MCP - 1和IP - 10。玻璃体液样本中MCP - 1水平显著高于血清样本（p < 0.001）。与RD患者相比，PVR和PDR患者玻璃体液样本中的MCP - 1水平显著更高（p = 0.0002）。活动性PDR患者玻璃体液样本中的MCP - 1水平显著高于非活动性PDR患者（p = 0.0224）。玻璃体液样本中IP - 10水平显著高于血清样本（p = 0.0035）。与RD患者相比，PVR和PDR患者玻璃体液样本中的IP - 10水平显著更高（p = 0.0083）。活动性PDR病例玻璃体液样本中IP - 10检测的发生率显著高于非活动性病例（p = 0.0214）。在所有患者以及RD和PDR患者的玻璃体液样本中，IP - 10检测的发生率与MCP - 1水平升高之间存在显著关联（所有比较p < 0.001）。MCP - 1和SDF - 1定位于PVR和PDR膜中的肌成纤维细胞以及PDR膜中的血管内皮细胞。CXCR3由PDR膜中的血管内皮细胞表达。