[表皮生长因子诱导培养牛小梁细胞中c-fos基因的表达]
[The expression of c-fos gene in cultured bovine trabecular cells induced by epidermal growth factor].
作者信息
Yang X, Li M
机构信息
Department of Ophthalmology, First Hospital, Beijing Medical University.
出版信息
Zhonghua Yan Ke Za Zhi. 1997 Sep;33(5):370-2.
OBJECTIVE
To investigate the effect of epidermal growth factor (EGF) on cultured trabecular cells.
METHODS
Molecular hybridization technique was used to investigate the effect of EGF on c-fos expression and its induction of bovine trabecular cells in vitro, and 3H-TdR incorporation method was applied to study the synthesis of DNA.
RESULTS
EGF enhanced the incorporation of 3H-TdR into trabecular cells at concentrations ranging from 20-150 ng/ml (P < 0.001). Compared with control, the expression of c-fos appeared at 0.5 hour and 1 hour after stimulation, and did not exist till 2 hr. The level of c-fos expression in cells relied on the concentration of EGF.
CONCLUSION
Our results suggest that EGF stimulate trabecular cell proliferation at least in part by the regulation of the c-fos gene and its nuclear protein.
目的
研究表皮生长因子（EGF）对培养的小梁细胞的作用。
方法
采用分子杂交技术研究EGF对体外培养的牛小梁细胞中c-fos表达及其诱导作用，并用3H-TdR掺入法研究DNA的合成。
结果
在20-150 ng/ml浓度范围内，EGF增强了3H-TdR掺入小梁细胞的能力（P < 0.001）。与对照组相比，c-fos的表达在刺激后0.5小时和1小时出现，2小时后消失。细胞中c-fos的表达水平依赖于EGF的浓度。
结论
我们的结果表明，EGF至少部分通过调节c-fos基因及其核蛋白来刺激小梁细胞增殖。
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