[通过体外测定牛小梁细胞中3',5'-环磷酸腺苷来确定肾上腺素能拮抗剂对肾上腺素的影响]

[The effects of adrenergic antagonists on epinephrine determined by the assay of adenosine 3',5'-monophosphate in bovine trabecular cells in vitro].

作者信息

Lu Y, Li M, Shen Y

机构信息

Department of Ophthalmology, Xuanwu Hospital, Beijing.

出版信息

Zhonghua Yan Ke Za Zhi. 1997 Sep;33(5):373-5.

PMID:10451986

Abstract

OBJECTIVE

To compare and assay the effects of various beta-adrenergic antagonists at effective concentrations on epinephrine (EPI).

METHOD

Cyclic adenosine 3',5'-monophosphate (cAMP) in trabecular cells was measured by protein binding assay with 3H-cAMP.

RESULTS

(1) The effect of EPI increasing cAMP in bovine trabecular cells (BTC) in vitro did not obviously recover at the fifth hour after the removal of 10(-5) mol/L timolol, and partly recovered at the fifth hour after the removal of 10(-6) mol/L timolol. (2) There was only part recovery for the effect of EPI at the fifth hour after the removal of ICI 118, 551 (10(-5) mol/L, and 10(-6) mol/L). (3) There was an obvious recovery for the effect of EPI at the fifteenth minute after the removal of 10(-5) mol/L bisoprolol.

CONCLUSION

The effect of timolol and ICI 118, 551 for blocking EPI is strong and sustained; bisoprolol produces a smaller blockade to the effect of EPI, lasting a short time. Our results also presume that BTC contains beta-adrenergic receptors, and beta 2-adrenergic receptors are dominant.

摘要

目的

比较并测定不同有效浓度的β-肾上腺素能拮抗剂对肾上腺素（EPI）的作用。

方法

采用³H-cAMP蛋白结合分析法测定小梁细胞中的环磷酸腺苷（cAMP）。

结果

（1）在去除10⁻⁵mol/L噻吗洛尔后第5小时，EPI在体外增加牛小梁细胞（BTC）中cAMP的作用未明显恢复，而在去除10⁻⁶mol/L噻吗洛尔后第5小时部分恢复。（2）在去除ICI 118,551（10⁻⁵mol/L和10⁻⁶mol/L）后第5小时，EPI的作用仅有部分恢复。（3）在去除10⁻⁵mol/L比索洛尔后第15分钟，EPI的作用有明显恢复。