Mur C, Martínez-Carpio P A, Fernández-Montolí M E, Ramon J M, Rosel P, Navarro M A
Hormone Unit, Biochemistry Department, Ciutat Sanitària i Universitària de Bellvitge, Barcelona, Spain.
Cell Biol Int. 1998;22(9-10):679-84. doi: 10.1006/cbir.1998.0306.
The human cell line MDA-MB-231 is a prototype for the study of hormone-independent breast cancer. Modification of cell growth behaviour has been observed after treating these cells with growth factors. EGF is a typical stimulatory growth factor for many cell types, whereas transforming growth factor beta(1)(TGF-beta(1)) acts with inhibitory character. Here we observed cell growth inhibition after EGF as well as after TGF-beta(1)treatments. Nevertheless, in the 42-h experiments, EGF-treated cultures grew before (18 hours) respect to the TGF-beta(1)and E(2)-treated cultures (24 h), and in the 11-day experiments, EGF-treated cultures started growing (7 days) after TGF-beta(1)-treated cultures (5 days). Estradiol inhibited the proliferation of these cells only after several days of treatment.
人细胞系MDA-MB-231是激素非依赖性乳腺癌研究的一个原型。在用生长因子处理这些细胞后，已观察到细胞生长行为的改变。表皮生长因子（EGF）是许多细胞类型的典型刺激性生长因子，而转化生长因子β(1)（TGF-β(1)）具有抑制作用。在这里，我们观察到EGF以及TGF-β(1)处理后细胞生长受到抑制。然而，在42小时的实验中，EGF处理的培养物在（18小时）比TGF-β(1)和雌二醇（E(2)）处理的培养物（24小时）更早开始生长，并且在11天的实验中，EGF处理的培养物在TGF-β(1)处理的培养物（5天）之后（7天）开始生长。雌二醇仅在处理几天后才抑制这些细胞的增殖。
