Ishii Y, Asai S, Kohno T, Suzuki S, Ishii M, Hosoi I, Fujii M, Iwai S, Ishikawa K
Department of Pharmacology, 3rd Department of Surgery, Nihon University School of Medicine, Itabashi-ku, Tokyo, 173, Japan.
J Surg Res. 1999 Sep;86(1):130-5. doi: 10.1006/jsre.1999.5705.
Using a rat model of hepatectomy, we investigated whether the severity of hepatopathy could be quantitatively measured from changes in expiratory (13)CO(2) levels after intravenous administration of L-[1-(13)C]methionine or L-[1-(13)C]phenylalanine.
Under nembutal anesthesia, 30 mg/kg L-[1-(13)C]phenylalanine or 40 mg/kg L-[1-(13)C]methionine was administered to rats through the femoral vein, and expiratory (13)CO(2) levels were measured for 15 min. A 30, 70, or 90% hepatectomy was performed. In the control group, simple laparotomy was performed. Breath test was conducted 20 min after laparotomy. We examined the correlation of the total (13)CO(2) output over 15 min or peak (13)CO(2) level with liver weight/body weight (%).
In breath test graphs, L-[1-(13)C]methionine did not show any peak level during measurement. L-[1-(13)C]phenylalanine showed a specific peak level 6 +/- 1 min after administration. The correlation coefficient between total (13)CO(2) output over 15 min after L-[1-(13)C]methionine administration and liver weight/body weight was 0.922 (P < 0.001). The correlation coefficient between total (13)CO(2) output over 15 min after L-[1-(13)C]phenylalanine administration and liver weight/body weight was 0.883 (P < 0.001). The correlation coefficient between peak L-[1-(13)C]phenylalanine level and liver weight/body weight was highest, 0.927 (P < 0.001).
In a breath test with intravenously administered L-[1-(13)C]methionine or L-[1-(13)C]phenylalanine, hepatopathy could be quantitatively evaluated by measuring expiratory (13)CO(2) levels over 15 min. After administration of L-[1-(13)C]phenylalanine, hepatopathy could be quantitatively evaluated in a short period by measuring the peak expiratory (13)CO(2) level.
我们使用肝切除术大鼠模型，研究静脉注射L-[1-(13)C]蛋氨酸或L-[1-(13)C]苯丙氨酸后，是否可通过呼气(13)CO(2)水平的变化对肝病严重程度进行定量测量。
在戊巴比妥麻醉下，通过股静脉给大鼠注射30mg/kg的L-[1-(13)C]苯丙氨酸或40mg/kg的L-[1-(13)C]蛋氨酸，并测量15分钟的呼气(13)CO(2)水平。进行30%、70%或90%的肝切除术。对照组进行单纯剖腹术。剖腹术后20分钟进行呼气试验。我们检查了15分钟内的总(13)CO(2)排出量或(13)CO(2)峰值水平与肝重/体重(%)之间的相关性。
在呼气试验图中，L-[1-(13)C]蛋氨酸在测量期间未显示任何峰值水平。L-[1-(13)C]苯丙氨酸在给药后6±1分钟显示出特定的峰值水平。L-[1-(13)C]蛋氨酸给药后15分钟内的总(13)CO(2)排出量与肝重/体重之间的相关系数为0.922(P<0.001)。L-[1-(13)C]苯丙氨酸给药后15分钟内的总(13)CO(2)排出量与肝重/体重之间的相关系数为0.883(P<0.001)。L-[1-(13)C]苯丙氨酸峰值水平与肝重/体重之间的相关系数最高，为0.927(P<0.001)。
在静脉注射L-[1-(13)C]蛋氨酸或L-[1-(13)C]苯丙氨酸的呼气试验中，通过测量15分钟内的呼气(13)CO(2)水平可对肝病进行定量评估。注射L-[1-(13)C]苯丙氨酸后，通过测量呼气(13)CO(2)峰值水平可在短时间内对肝病进行定量评估。
