Lin J, Li L, Gao Y, Min B, Xu X
Institute of Basic Medical Sciences, CAMS, Beijing.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1997 Feb;19(1):24-8.
We determined the cytokine messenger RNA (mRNA) expression patterns of peripheral blood mononuclear cells from 17 patients with multiple sclerosis (MS) and 11 normal controls, by using a quantitative reverse transcription in combination with polymerase reaction (RT/PCR) and Southern blot hybridization method. A significantly higher levels of IL-2, IFN-gamma mRNA in patient with MS compared to normal controls and significantly higher level of expressions of IFN-gamma, TNF-alpha mRNA were detected in active MS patients compared to those with stable desease (P < 0.05). These data further support the central role of some cytokines in the pathophysiology of this autoimmune disease.
我们采用定量逆转录结合聚合酶反应（RT/PCR）和Southern印迹杂交方法，测定了17例多发性硬化症（MS）患者和11名正常对照者外周血单个核细胞的细胞因子信使核糖核酸（mRNA）表达模式。与正常对照相比，MS患者的IL-2、IFN-γ mRNA水平显著更高；与病情稳定的患者相比，活动期MS患者的IFN-γ、TNF-α mRNA表达水平显著更高（P < 0.05）。这些数据进一步支持了某些细胞因子在这种自身免疫性疾病病理生理学中的核心作用。
