[cDNA cloning of human stem cell factor and its high level expression in E. coli].
作者信息
Chen W, Di X, Li J, Song F, Chen S
机构信息
Institute of Basic Medical Sciences, CAMS, Beijing.
出版信息
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1997 Feb;19(1):29-34.
PMID:10453549
Abstract
The total RNA of HepG2 cell was extracted as the template by ultrocentrifuge method. The full length cDNA (0.8 kb) encoding the human stem cell factor (hSCF) was amplified by RT-PCR method. The cDNA encoding mature hSCF (0.5 kb) was sequenced and was recombined into the expression vector (PBV-220). The expression level of rhSCF in E. coli DH5 alpha was about 15% of the total protein.
摘要
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