[Effect of calcium and angiotensin II receptor on angiotensin II-stimulated protein synthesis in neonatal myocardial cells].

In this study, the role of angiotensin II (Ang II) receptor, Ca2+ in the Ang II-stimulated neoeatal myocytes protein synthesis were investigated and the effect of Ang II on expression of c-fos gene was observed. The results showed that the stimulatory effect of Ang II on protein synthesis was blocked by the Ang II antagonist-[Sar1 Ile8] Ang II. When verapamil, EG-TA, dantrolene, and Fura-2/AM, were added to the medium, the 3H-leucine incorporations stimulated by Ang II were reduced by 17%, 19%, 22%, and 27%. Using calcium ionophore-A23187 did not stimulate protein synthesis, but enhanced Ang II-induced 3H-leucine incorporation. In addition, Ang II could induce expression of c-fos gene. These results indicated that the hypertrophic effect of Ang II on the myocytes was mediated by Ang II receptor and depended on increase in [Ca2+]i and may be associated with enhanced expression of c-fos.