Zhang X, Jiang M, Chui J, Wang H, Ma J
PUMC Hospital, Beijing.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1997 Aug;19(4):284-7.
To investigate the clinical significance of antineutrophil cytoplasmic antibodies (ANCA) in Wegener's granulomatosis (WG) and other diseases.
Indirect immunofluorescence was applied to detect serum ANCA of 204 patients and 48 normal controls. Enzyme-linked immunosorbent assay (ELISA) was used to detect antimyeloperoxidase (MPO) antibodies.
The frequency of ANCA in sera of WG patients was 75% and significantly higher than those in sera of other groups (P < 0.05). ELISA demonstrated that MPO was the major antigen recognized by perinuclear ANCA (P-ANCA). Cytoplasmic ANCA (C-ANCA) was always positive and high titers in sera of active WG patients, but turned to negative or low titers in remission.
These results suggested that C-ANCA was specific for WG and was a marker for disease activity in WG. P-ANCA wasn't specific for WG, but related to vasculitis.
探讨抗中性粒细胞胞浆抗体（ANCA）在韦格纳肉芽肿（WG）及其他疾病中的临床意义。
采用间接免疫荧光法检测204例患者及48例正常对照者血清中的ANCA。采用酶联免疫吸附测定（ELISA）法检测抗髓过氧化物酶（MPO）抗体。
WG患者血清中ANCA的阳性率为75%，显著高于其他组（P<0.05）。ELISA结果显示，MPO是核周型ANCA（P-ANCA）识别的主要抗原。胞浆型ANCA（C-ANCA）在活动期WG患者血清中始终呈阳性且滴度较高，但在缓解期转为阴性或低滴度。
这些结果提示，C-ANCA对WG具有特异性，是WG疾病活动的标志物。P-ANCA对WG不具有特异性，但与血管炎有关。
