Luckas M J, Taggart M J, Wray S
Department of Obstetrics and Gynecology, University of Liverpool, Manchester, United Kingdom.
Am J Obstet Gynecol. 1999 Aug;181(2):468-76. doi: 10.1016/s0002-9378(99)70580-6.
We hypothesized that the release of calcium from intracellular stores contributes to the contractions produced by the agonists oxytocin, carbachol, and prostaglandin F(2 )(alpha ) in human myometrium.
Strips of myometrium were obtained at cesarean section and hysterectomy. The strips were loaded with the calcium-sensitive dye Indo-1 to enable simultaneous measurement of tension and intracellular calcium levels. Agonist-induced responses in the presence and absence of extracellular calcium were studied.
Strips of myometrium were obtained from 48 women not in labor undergoing cesarean section and 6 women not pregnant undergoing hysterectomy. An increase in intracellular calcium level after agonist stimulation invariably preceded an increase in tension. Intracellular calcium level returned to baseline before myometrial relaxation. Oxytocin, carbachol, and prostaglandin F(2)(alpha) all gave both force and intracellular calcium responses in the absence of extracellular calcium, although both these responses were only 26% to 40% of the maximal response when extracellular calcium was present.
Release of calcium from internal stores induced by oxytocin, carbachol, and prostaglandin F(2)(alpha) may contribute to agonist-induced myometrial force production.
我们推测细胞内钙库释放的钙有助于催产素、卡巴胆碱和前列腺素F₂α在人子宫肌层产生的收缩。
在剖宫产和子宫切除术中获取子宫肌层条带。将这些条带用钙敏染料Indo-1加载，以便同时测量张力和细胞内钙水平。研究了在有和没有细胞外钙的情况下激动剂诱导的反应。
从48例未临产行剖宫产的妇女和6例未孕行子宫切除术的妇女中获取子宫肌层条带。激动剂刺激后细胞内钙水平的升高总是先于张力的增加。在子宫肌层松弛之前，细胞内钙水平恢复到基线。催产素、卡巴胆碱和前列腺素F₂α在没有细胞外钙的情况下均产生了力和细胞内钙反应，尽管这两种反应仅为有细胞外钙时最大反应的26%至40%。
催产素、卡巴胆碱和前列腺素F₂α诱导的细胞内钙库释放的钙可能有助于激动剂诱导的子宫肌层产力。
