奇异变形杆菌O24和O29的O-特异性多糖结构及脂多糖的血清学交叉反应性

Structures of the O-specific polysaccharides and a serological cross-reactivity of the lipopolysaccharides of Proteus mirabilis O24 and O29.

作者信息

Literacka E, Perepelov A V, Senchenkova S N, Zatonsky G V, Shashkov A S, Knirel Y A, Kaca W

机构信息

Institute of Microbiology and Immunology, University of Lodz, Poland.

出版信息

FEBS Lett. 1999 Aug 6;456(2):227-31. doi: 10.1016/s0014-5793(99)00952-7.

DOI:10.1016/s0014-5793(99)00952-7

PMID:10456314

Abstract

Strains of Proteus mirabilis belonging to serogroups O24 and O29 are frequent in clinical specimens. Anti-P. mirabilis O24 serum cross-reacted with the lipopolysaccharide (LPS) of P. mirabilis O29 and vice versa. The structures of the O-specific polysaccharides (OPSs, O-antigens) of both LPSs were established using sugar analysis and one- and two-dimensional 1H- and 13C-NMR spectroscopy and found to be different. SDS-PAGE and Western immunoblotting suggested that the serological cross-reactivity of the LPSs is due to a common epitope(s) on the core-lipid A moiety, rather than on the OPS. Therefore, the epitope specificity and the structures of the O-antigens studied are unique among Proteus serogroups.

摘要

奇异变形杆菌O24和O29血清群的菌株在临床标本中很常见。抗奇异变形杆菌O24血清与奇异变形杆菌O29的脂多糖（LPS）发生交叉反应，反之亦然。利用糖分析以及一维和二维1H-和13C-NMR光谱确定了两种LPS的O-特异性多糖（OPS，O-抗原）结构，发现它们不同。SDS-PAGE和Western免疫印迹表明，LPS的血清学交叉反应性是由于核心脂质A部分上存在共同表位，而非OPS上。因此，所研究的O-抗原的表位特异性和结构在变形杆菌血清群中是独特的。