奇异变形杆菌O23和普通变形杆菌O23 O抗原的免疫化学研究。
Immunochemical studies on the O-antigens of Proteus mirabilis O23 and Proteus vulgaris O23.
作者信息
Rózalski Antoni, Perepelov Andrei V, Bartodziejska Beata, Senchenkova Sof'ya N, Babicka Dorota, Kaca Wiesław, Knirel Yuriy A
机构信息
Department of Immunobiology of Bacteria, Institute of Microbiology and Immunology, University of Lódź, Banacha 12/16, 90-237 Lódź, Poland.
出版信息
Arch Immunol Ther Exp (Warsz). 2003;51(1):69-74.
Analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy demonstrated that the O-specific polysaccharides of Proteus mirabilis PrK 42/57 and P. vulgaris PrK 43/57 are structurally similar to that of P. vulgaris PrK 44/57 and different from the polysaccharide of P. mirabilis PrK 41/57 studied earlier. The lipopolysaccharides of these strains were tested using enzyme immunosorbent assay, passive hemolysis and Western blot with O-antisera against P. mirabilis 42/57 and P. vulgaris 43/57 and 44/57, as well as with cross-absorbed O-antisera. The chemical and serological data revealed the basis for combining the four strains into Proteus serogroup O23 and division of this serogroup to three subgroups, one for P. vulgaris 43/57 and 44/57 and two others for P. mirabilis 41/57 and 42/57.
通过氢-1（1H）和碳-13（13C）核磁共振（NMR）光谱分析表明，奇异变形杆菌PrK 42/57和普通变形杆菌PrK 43/57的O-特异性多糖在结构上与普通变形杆菌PrK 44/57的相似，而与先前研究的奇异变形杆菌PrK 41/57的多糖不同。使用酶联免疫吸附测定、被动溶血以及针对奇异变形杆菌42/57和普通变形杆菌43/57及44/57的O-抗血清以及交叉吸收的O-抗血清进行蛋白质印迹法，对这些菌株的脂多糖进行了检测。化学和血清学数据揭示了将这四个菌株归为变形杆菌O23血清群以及将该血清群分为三个亚群的依据，一个亚群包括普通变形杆菌43/57和44/57，另外两个亚群分别包括奇异变形杆菌41/57和42/57。
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