单倍体生殖细胞特异性微管蛋白cDNA的分子克隆及小鼠睾丸中该蛋白的分析。
Molecular cloning of haploid germ cell-specific tektin cDNA and analysis of the protein in mouse testis.
作者信息
Iguchi N, Tanaka H, Fujii T, Tamura K, Kaneko Y, Nojima H, Nishimune Y
机构信息
Department of Science for Laboratory Animal Experimentation, Research Institute for Microbial Diseases, Osaka University, Suita City, Japan.
出版信息
FEBS Lett. 1999 Aug 6;456(2):315-21. doi: 10.1016/s0014-5793(99)00967-9.
Tektins are a class of proteins that form filamentous polymers in the walls of ciliary and flagellar microtubules. We report here the molecular cloning of a new member of the tektin family, tektin-t, identified from a mouse haploid germ cell-specific cDNA library. Tektin-t mRNA encodes a protein of 430 deduced amino acids possessing RSNVELCRD, the conserved sequence of tektin family proteins. Western blotting showed a single band having a molecular weight of 86 kDa in the mouse testis. Immunohistochemistry of the testis showed that tektin-t is localized in the flagella of elongating spermatids from developmental step 15 to maturity.
纤丝蛋白是一类在纤毛和鞭毛微管壁中形成丝状聚合物的蛋白质。我们在此报告从一个小鼠单倍体生殖细胞特异性cDNA文库中鉴定出的纤丝蛋白家族新成员——纤丝蛋白-t的分子克隆。纤丝蛋白-t mRNA编码一种由430个推导氨基酸组成的蛋白质，该蛋白质具有纤丝蛋白家族蛋白质的保守序列RSNVELCRD。蛋白质免疫印迹法显示在小鼠睾丸中有一条分子量为86 kDa的单条带。睾丸的免疫组织化学显示，纤丝蛋白-t定位于从发育第15阶段到成熟阶段的伸长精子细胞的鞭毛中。
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