Pepino M Y, López M F, Spear N E, Molina J C
Instituto de Investigación Médica Mercedes y Martín Ferreyra, Córdoba, Argentina.
Alcohol. 1999 Jun-Jul;18(2-3):189-201. doi: 10.1016/s0741-8329(99)00003-8.
Our previous studies indicate that rat pups are able to detect the low levels of ethanol (175 mg %) found in the milk of a moderately intoxicated dam. The present study tested the effect of infantile interactions (including suckling) with ethanol-treated mothers on later behavioral responsiveness to ethanol's sensory properties. In Experiment 1, pups suckled from dams subjected to a 2.5 g/kg ethanol dose (i.g.) or water-treated females during postnatal days (PDs) 3, 5, 7, 9, 11, and 13. During PD 15, these pups were exposed to procedures to induce a conditioned aversion to the low level of ethanol (175 mg % in water), with lithium chloride as the unconditioned stimulus. Conditioning was more effective for pups with the prior ethanol experience within the nursing context. Greater responsiveness to ethanol in milk also was found for conditioning control pups that had interacted with intoxicated dams than for those that had interacted with water-treated dams. Experiment 2 determined that interaction with an intoxicated dam was sufficient for altered responsiveness to ethanol, in that the additional conditioning procedures of Experiment 1 were not needed for the effect. Generally, a relatively brief history of infantile interaction with ethanol-intoxicated dams increased later responsiveness to ethanol's orosensory properties. The results suggest that moderately intoxicated dams within the nursing context provide information to the progeny that may lead to the establishment of ethanol-related memories.
我们之前的研究表明，幼鼠能够检测到中度醉酒母鼠乳汁中低水平的乙醇（175毫克%）。本研究测试了与经乙醇处理的母亲进行婴儿期互动（包括哺乳）对后期对乙醇感官特性行为反应的影响。在实验1中，幼鼠在出生后第3、5、7、9、11和13天从接受2.5克/千克乙醇剂量（腹腔注射）的母鼠或接受水处理的母鼠处哺乳。在出生后第15天，这些幼鼠接受诱导对低水平乙醇（水中175毫克%）产生条件性厌恶的程序，以氯化锂作为非条件刺激。在哺乳环境中，先前有乙醇经历的幼鼠的条件反射更有效。与与水处理母鼠互动的条件对照幼鼠相比，与醉酒母鼠互动的条件对照幼鼠对乳汁中乙醇的反应性也更高。实验2确定，与醉酒母鼠的互动足以改变对乙醇的反应性，因为实验1的额外条件反射程序对这种效应不是必需的。一般来说，与乙醇中毒母鼠进行相对短暂的婴儿期互动史会增加后期对乙醇口腔感觉特性的反应性。结果表明，哺乳环境中的中度醉酒母鼠会向后代提供信息，这可能导致与乙醇相关记忆的形成。
