一种针对脑膜炎奈瑟菌NspA外膜蛋白的单克隆抗体的杀菌及交叉保护活性
Bactericidal and cross-protective activities of a monoclonal antibody directed against Neisseria meningitidis NspA outer membrane protein.
作者信息
Cadieux N, Plante M, Rioux C R, Hamel J, Brodeur B R, Martin D
机构信息
Unité de Recherche en Vaccinologie, Centre Hospitalier Universitaire de Québec et Université Laval, Ste-Foy, Québec, Canada G1V 4G2.
出版信息
Infect Immun. 1999 Sep;67(9):4955-9. doi: 10.1128/IAI.67.9.4955-4959.1999.
Abstract
The cross-bactericidal and cross-protective activities of a monoclonal antibody (MAb) named Me-7, which is directed against an antigenically highly conserved epitope on the meningococcal NspA protein, were studied. This MAb efficiently killed in vitro, in the presence of rabbit or human serum, 13 of 14 meningococcal strains tested, including 9 of 9, 2 of 3, and 2 of 2 strains of serotypes B, A, and C, respectively. MAb Me-7 also significantly reduced by more than 75% the levels of bacteremia recorded for mice challenged with 10 of 11 meningococcal strains tested. Analysis of the predicted amino acid sequence of the NspA protein from the meningococcal strain MCH88 (A:4:P1.10), which was not killed by MAb Me-7, indicated the presence of an additional glutamine residue at position 73, compared to the three other NspA sequences. The data presented in this study suggest that antibodies directed against this highly conserved outer membrane protein could protect against meningococcal infections.
摘要
研究了一种名为Me-7的单克隆抗体（MAb）的交叉杀菌和交叉保护活性，该抗体针对脑膜炎球菌NspA蛋白上一个抗原性高度保守的表位。在兔血清或人血清存在的情况下，这种单克隆抗体在体外有效杀灭了所检测的14株脑膜炎球菌中的13株，分别包括9株B血清型、3株A血清型中的2株以及2株C血清型中的2株。MAb Me-7还使接受所检测的11株脑膜炎球菌中10株攻击的小鼠的菌血症水平显著降低了75%以上。对未被MAb Me-7杀灭的脑膜炎球菌菌株MCH88（A:4:P1.10）的NspA蛋白预测氨基酸序列分析表明，与其他三个NspA序列相比，在第73位存在一个额外的谷氨酰胺残基。本研究提供的数据表明，针对这种高度保守的外膜蛋白的抗体可以预防脑膜炎球菌感染。
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Highly conserved Neisseria meningitidis surface protein confers protection against experimental infection.高度保守的脑膜炎奈瑟菌表面蛋白可提供针对实验性感染的保护。
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