Amano Y, Okumura C, Yoshida M, Katayama H, Unten S, Arai J, Tagawa T, Hoshina S, Hashimoto H, Ishikawa H
Daikin Industries, Ltd.
Hum Cell. 1999 Mar;12(1):3-10.
New trend in methods for assessing pharmacological action to bacteria and cell is to measure their metabolic activities induced, while the conventional methods used population growth. We focused on respiration volume as an indicator of cell metabolism, and developed inexpensive disposable oxygen electrode sensor and multi-channel dissolved oxygen meters (DOX-10 and DOX-96KB). Using these instruments, cytotoxicity was measured for 48 hrs and the method showed superior features to conventional methods in its handiness of one step assay, and excellent adaptability to automated systems. Total usability of this oxygen electrode method is being evaluated in bacterial drug susceptibility test, anticancer drug susceptibility test, and alternatives to animal experiment.
评估药物对细菌和细胞药理作用的新趋势是测量它们诱导的代谢活性，而传统方法是使用群体生长情况。我们将呼吸量作为细胞代谢的指标，并开发了廉价的一次性氧电极传感器和多通道溶解氧仪（DOX - 10和DOX - 96KB）。使用这些仪器，对细胞毒性进行了48小时的测量，该方法在一步测定的便利性以及对自动化系统的出色适应性方面显示出优于传统方法的特性。这种氧电极方法的整体实用性正在细菌药敏试验、抗癌药敏试验以及动物实验替代方法中进行评估。
