Oral and Maxillofacial Surgery and Systemic Medicine, Graduate School of Life Dentistry at Niigata, Japan.
Hum Cell. 2010 Aug;23(3):103-12. doi: 10.1111/j.1749-0774.2010.00092.x.
Conventional anticancer drug sensitivity testing methods, such as succinate dehydrogenase inhibition (SDI), histoculture drug-response assay (HDRA) and collagen gel droplet embedded culture drug sensitivity testing (CD-DST), all require primary culturing and are extremely complex tests that require considerable time for analysis. A major drawback of these methods is that if culturing is not performed properly, ambiguous results are produced. Therefore, we developed an oxygen electrode apparatus that uses cellular metabolism as an indicator of anticancer drug sensitivity and investigated its usefulness in 29 breast cancer patients with the following histopathological classifications: papillotubular carcinoma (n= 15); solid tubular carcinoma (n= 6); and scirrhous carcinoma (n= 8). Comparison of anticancer drug sensitivity testing results obtained using the conventional HDRA method and those obtained using the oxygen electrode apparatus showed significant reproducibility between the two methods. In addition, similar anticancer drug sensitivity testing results using the oxygen electrode apparatus were obtained for in vivo testing of nude mice transplanted with established cancer cell lines. These findings suggest that the oxygen electrode apparatus is a useful procedure in anticancer drug sensitivity testing that provides better reproducibility and that is faster, more convenient, and less expensive than other testing methods.
传统的抗癌药物敏感性测试方法，如琥珀酸脱氢酶抑制（SDI）、组织培养药物反应测定（HDRA）和胶原凝胶液滴包埋培养药物敏感性测试（CD-DST），都需要进行原代培养，且这些方法极其复杂，分析需要相当长的时间。这些方法的一个主要缺点是，如果培养不当，会产生模棱两可的结果。因此，我们开发了一种使用细胞代谢作为抗癌药物敏感性指标的氧电极装置，并在 29 名具有以下组织病理学分类的乳腺癌患者中研究了其用途：乳头状管状癌（n=15）；实体管状癌（n=6）；和硬癌（n=8）。使用传统的 HDRA 方法和氧电极装置获得的抗癌药物敏感性测试结果进行比较，两种方法之间具有显著的重现性。此外，使用氧电极装置对建立的癌细胞系移植的裸鼠进行体内测试，得到了相似的抗癌药物敏感性测试结果。这些发现表明，氧电极装置是一种有用的抗癌药物敏感性测试程序，它提供了更好的重现性，并且比其他测试方法更快、更方便、更经济。
