Hayashi J
Department of Endocrinology and Metabolism, Tokyo Metropolitan Geriatric Hospital, Japan.
Hum Cell. 1999 Mar;12(1):31-5.
A quantitative angiogenesis in vitro was investigated by culturing bovine carotid artery endothelial cells between two layers of type I collagen gel. Cells become organized into tube-like structures within few days. Ultrastructurally, tubular structures were composed of one to several endothelial cells containing pinocytotic vesicles and cytoplasmic projections, and linked by junctional complexes. A basal lamina-like structure surrounded the abluminal surface. Glucose, insulin, insulin-like growth factor I at pathophysiological high concentrations significantly stimulated tube-forming activity of endothelial cells by stimulating cell migration.
通过在两层I型胶原凝胶之间培养牛颈动脉内皮细胞，对体外定量血管生成进行了研究。细胞在几天内就组织成管状结构。超微结构上，管状结构由一到几个含有吞饮小泡和细胞质突起的内皮细胞组成，并通过连接复合体相连。基底膜样结构围绕着管腔外表面。病理生理高浓度的葡萄糖、胰岛素、胰岛素样生长因子I通过刺激细胞迁移显著刺激内皮细胞的管形成活性。
