Murphy E F, Keddie J L, Lu J R, Brewer J, Russell J
School of Physical Sciences, University of Surrey, Guildford, UK.
Biomaterials. 1999 Aug;20(16):1501-11. doi: 10.1016/s0142-9612(99)00059-9.
Coating hydrogel polymers onto solid substrates can reduce the adsorption of proteins onto these surfaces, but the extent of the reduction in protein adsorption is strongly dependent on how the surface layer is coated. We have examined the effect of coating conditions on the structure of thin polymer films formed from a number of poly(methacrylate)-based hydrogel polymers via the dip-coating method. We show in this work how the polarity of the solvent, the speed of lifting, and the annealing temperature affect the thickness and uniformity of ultrathin phosphorylcholine (PC)-incorporated polymer films coated on the surface of native oxide on silicon and the subsequent interaction of these coated surfaces with lysozyme molecules. Our results show that the uniformity of the polymer film, and thus the smoothness of the outer film surface, influence the extent of reduction in protein adsorption. We suggest that the reduction in lysozyme adsorption is the result of a layer of PC groups on the surface of the polymer film. The improvement of the smoothness of the film results in the formation of a close-packed PC layer on the outer surface of the polymer film, leaving few defects or cavities on which protein molecules can bind.
将水凝胶聚合物涂覆在固体基质上可以减少蛋白质在这些表面上的吸附，但蛋白质吸附减少的程度在很大程度上取决于表面层的涂覆方式。我们通过浸涂法研究了涂覆条件对由多种聚（甲基丙烯酸酯）基水凝胶聚合物形成的聚合物薄膜结构的影响。在这项工作中，我们展示了溶剂的极性、提拉速度和退火温度如何影响涂覆在硅原生氧化物表面的超薄含磷酰胆碱（PC）聚合物薄膜的厚度和均匀性，以及这些涂覆表面随后与溶菌酶分子的相互作用。我们的结果表明，聚合物薄膜的均匀性，进而外膜表面的光滑度，会影响蛋白质吸附减少的程度。我们认为溶菌酶吸附的减少是聚合物薄膜表面一层PC基团的结果。薄膜光滑度的提高导致在聚合物薄膜外表面形成紧密堆积的PC层，几乎没有可供蛋白质分子结合的缺陷或空洞。
