Numazaki K, Asanuma H
Department of Pediatrics, Sapporo Medical University, School of Medicine, Japan.
In Vivo. 1999 May-Jun;13(3):239-41.
Anti-human cytomegalovirus (HCMV) properties of povidone-iodine (PVP-I) were evaluated in vitro. The effect of PVP-I was evaluated by indirect immunofluorescence assay on HCMV-infected MRC-5 cells. Percentages of fluorescent cells positive for HCMV immediate early and early antigens in cultures inoculated with AD 169 treated with PVP-I at various concentrations and reaction times were compared with the number of fluorescent cells in the controls inoculated with the virus alone. PVP-I was found to exert some inhibitory effect at a concentration of 0.5% and complete inhibition at a concentration of 7.5% on the infection of MRC-5 cells by HCMV AD 169 strain. Entirely new approaches to the prevention of HCMV infections in infants are necessary. The adequate use of PVP-I as a disinfectant may reduce the transmission of HCMV.
对聚维酮碘（PVP-I）的抗人巨细胞病毒（HCMV）特性进行了体外评估。通过间接免疫荧光法评估PVP-I对HCMV感染的MRC-5细胞的作用。将用不同浓度和反应时间的PVP-I处理的AD 169接种培养物中HCMV即刻早期和早期抗原阳性荧光细胞的百分比与仅接种病毒的对照中的荧光细胞数量进行比较。发现PVP-I在浓度为0.5%时对HCMV AD 169株感染MRC-5细胞有一定抑制作用，在浓度为7.5%时完全抑制。预防婴儿HCMV感染需要全新的方法。适当使用PVP-I作为消毒剂可能会减少HCMV的传播。
