Lee C J, Kim J K
Korea Atomic Energy Research Institute (KAERI), Taejon, Korea.
In Vivo. 1999 May-Jun;13(3):263-6.
We immunohistochemically examined the effect of gamma-radiation on immature mouse ovarian follicles. Mice were Jirradiated with a dose of LD80 (8.3 Gy) in KAERI. At 0 h, 6 h, 12 h, 1 d, 2 d, 4 d and 8 d postirradiation, the ovaries were excised and fixed in neutral buffered formalin. We performed immunohistochemistry for protein retinoblastoma (pRb), terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end-labeling (TUNEL) and routine hematoxylin-eosin staining in the largest cross sections. Radiation-induced follicular degeneration increased before 6 h, and most irradiated ovarian follicles became acutely atretic. The immunohistochemical staining for pRb was strong in the nuclei of granulosa cells of normal follicles and weak in atretic ones which were, conversely, strong for TUNEL staining. It was shown that pRb expression became lower with the degeneration of the ovarian follicles, which was inhibited by gamma-radiation. In the present study, pRb immunohistochemistry was proven to be a useful tool for the identification of follicular status.
我们通过免疫组织化学方法检测了γ射线对未成熟小鼠卵巢卵泡的影响。在韩国原子能研究所，用8.3 Gy的剂量对小鼠进行全身照射。照射后0小时、6小时、12小时、1天、2天、4天和8天，切除卵巢并固定于中性缓冲福尔马林中。我们在最大的横断面上对视网膜母细胞瘤蛋白（pRb）进行免疫组织化学检测、进行末端脱氧核苷酸转移酶介导的dUTP-地高辛配基缺口末端标记（TUNEL）以及常规苏木精-伊红染色。辐射诱导的卵泡退化在6小时前增加，大多数受照射的卵巢卵泡迅速闭锁。正常卵泡颗粒细胞核中pRb免疫组织化学染色强，而闭锁卵泡中弱，相反，闭锁卵泡TUNEL染色强。结果表明，随着卵巢卵泡退化，pRb表达降低，而γ射线可抑制这种降低。在本研究中，pRb免疫组织化学被证明是鉴定卵泡状态的有用工具。
