Morgan L, Foster F, Hayman R, Crawshaw S, Baker P N, Broughton Pipkin F, Kalsheker N
Clinical Chemistry Division, School of Clinical Laboratory Sciences, University Hospital, Nottingham, UK.
J Hypertens. 1999 Jun;17(6):765-8. doi: 10.1097/00004872-199917060-00007.
To investigate the hypothesis that pre-eclampsia is associated with a common insertion-deletion polymorphism in the angiotensin-converting enzyme gene.
Seventy-two women with pre-eclampsia and 83 normotensive pregnant women participated in the study. Pre-eclampsia was defined as a blood pressure exceeding 140/90 mm Hg in a previously normotensive woman, associated with proteinuria in excess of 300 mg/l in a 24 h collection. Samples for fetal genotyping were available from 66 pregnancies complicated by pre-eclampsia and 79 normotensive pregnancies.
Maternal and fetal samples were genotyped at the insertion-deletion (I-D) polymorphism in intron 16 of the angiotensin-converting enzyme gene by the polymerase chain reaction followed by agarose electrophoresis.
Neither the I-D genotype distributions nor the allele frequencies differed significantly between pre-eclamptic and normotensive pregnancies in maternal or fetal samples (phi2 <0.3, not significant). The odds ratio for pre-eclampsia in women with the DD genotype, compared with the ID and II genotype, was 1.09 (95% confidence interval 0.55-2.16). The odds ratio associated with the DD genotype in the fetus was 1.14 (0.56-2.32).
This study has found no evidence that the insertion-deletion polymorphism in the angiotensin-converting enzyme gene is associated with pre-eclampsia.
研究子痫前期与血管紧张素转换酶基因常见插入缺失多态性相关这一假说。
72例子痫前期妇女和83例血压正常的孕妇参与了该研究。子痫前期定义为既往血压正常的女性血压超过140/90 mmHg，且24小时尿蛋白定量超过300 mg/l。66例合并子痫前期的妊娠和79例血压正常的妊娠有可供进行胎儿基因分型的样本。
采用聚合酶链反应继以琼脂糖电泳法，对血管紧张素转换酶基因第16内含子的插入缺失（I-D）多态性进行母婴样本基因分型。
子痫前期组与血压正常组孕妇或胎儿样本的I-D基因型分布及等位基因频率均无显著差异（卡方<0.3，无统计学意义）。与ID和II基因型相比，DD基因型女性患子痫前期的比值比为1.09（95%置信区间0.55-2.16）。胎儿中与DD基因型相关的比值比为1.14（0.56-2.32）。
本研究未发现证据表明血管紧张素转换酶基因的插入缺失多态性与子痫前期相关。
