Ricci U, Giovannucci Uzielli M L, Klintschar M
Azienda Meyer - University of Florence, Human Genetics Service, Via Masaccio 209, I-50132 Florence, Italy.
Int J Legal Med. 1999;112(5):342-4. doi: 10.1007/s004140050267.
In this paper we describe a new primer pair for the short tandem repeat (STR) D12S391 which makes it possible to obtain considerably shorter amplification fragments (125-173 bp), compared to the previously published primers (205-253 bp). The primers were tested on 70 samples with known genotypes, and no differing results were found. In sensitivity studies, forensic casework samples and DNA quality studies, we proved that the new primers can improve the efficiency of the amplification. Moreover, the resolution of this locus on denaturing PAGE followed by silver staining was dramatically improved. This improvement was found to be most valuable for typing the rare.3 variants known for this locus. We also present and propose a new method for silver staining denaturing acrylamide gels.
在本文中，我们描述了一种用于短串联重复序列（STR）D12S391的新型引物对，与先前发表的引物（205 - 253 bp）相比，它能够获得明显更短的扩增片段（125 - 173 bp）。这些引物在70个已知基因型的样本上进行了测试，未发现不同结果。在灵敏度研究、法医案件样本和DNA质量研究中，我们证明了新引物可以提高扩增效率。此外，变性聚丙烯酰胺凝胶电泳（PAGE）后银染时该位点的分辨率得到了显著提高。发现这种改进对于鉴定该位点已知的罕见.3变体最有价值。我们还提出了一种用于银染变性丙烯酰胺凝胶的新方法。
