Wang T Y, Chen H S, Yang Y C, Tsou M C
Department of Pathology, Mackay Memorial Hospital, Taipei, Taiwan.
J Formos Med Assoc. 1999 Jul;98(7):500-5.
This study was conducted to compare the diagnoses and specimen adequacy of the ThinPrep (Cytyc Corporation, Boxborough, MA, USA) and conventional Papanicolaou preparation methods for cervical cytology. Cervical specimens from 972 patients from our gynecology clinic were analyzed. A single sample of the uterine cervix taken from each patient was first used to prepare the conventional Papanicolaou smear. Afterward, the residual tissue on the sampling device was rinsed into a fluid preservative from which two slides were prepared by means of the ThinPrep method. Conventional and ThinPrep slides were examined independently. Cytologic diagnoses and specimen adequacy were classified according to the Bethesda System. The diagnoses based on the ThinPrep and conventional smears matched in 939 (96.6%) cases. Low-grade squamous epithelial or higher grade lesions were detected with at least one of the methods in 59 cases and with both methods in 42 cases. Fifteen more cases of low-grade squamous epithelial or higher grade lesions (58 vs 43; p < 0.001) and 10 more cases of high-grade squamous epithelial lesions or carcinoma (42 vs 32; p = 0.006), were detected with ThinPrep than with conventional smears. The ThinPrep method yielded a higher rate of specimens that were satisfactory for cytologic examination than the conventional smears (89.8% vs 70.9%, p < 0.001). The detection rate of infectious agents was also higher with the ThinPrep method (14.9% vs 6.6%; p < 0.001). The reproducibility rate of the twin slides prepared with the ThinPrep method was greater than 99%. In conclusion, the ThinPrep method yielded a significantly higher positive detection rate of cervical abnormalities and more satisfactory specimens than conventional Papanicolaou smears.
本研究旨在比较ThinPrep（美国马萨诸塞州博克斯伯勒市Cytyc公司）和传统巴氏涂片法用于宫颈细胞学检查的诊断结果及标本充分性。对来自我们妇科诊所的972例患者的宫颈标本进行了分析。从每位患者采集的单个宫颈样本首先用于制备传统巴氏涂片。之后，将采样装置上的残留组织冲洗到液体保存剂中，再通过ThinPrep方法制备两张玻片。传统玻片和ThinPrep玻片独立进行检查。根据贝塞斯达系统对细胞学诊断和标本充分性进行分类。基于ThinPrep涂片和传统涂片的诊断在939例（96.6%）病例中相符。59例病例中至少有一种方法检测到低级别鳞状上皮或更高级别病变，42例病例两种方法均检测到。与传统涂片相比，ThinPrep检测出低级别鳞状上皮或更高级别病变的病例多15例（58例对43例；p<0.001），高级别鳞状上皮病变或癌的病例多10例（42例对32例；p = 0.006）。ThinPrep方法获得的细胞学检查满意标本率高于传统涂片（89.8%对70.9%，p<0.001）。ThinPrep方法检测感染因子的比率也更高（14.9%对6.6%；p<0.001）。用ThinPrep方法制备的双玻片的可重复性率大于99%。总之，与传统巴氏涂片相比，ThinPrep方法在宫颈异常的阳性检测率显著更高，且标本更令人满意。
