在硫还原细菌氧化醋酸脱硫单胞菌中存在氢化酶的首个证据。
First evidence for the presence of a hydrogenase in the sulfur-reducing bacterium Desulfuromonas acetoxidans.
作者信息
Brugna M, Nitschke W, Toci R, Bruschi M, Giudici-Orticoni M T
机构信息
Laboratoire de Bioénergétique et Ingénierie des Protéines, UPR 9036 CNRS, Institut de Biologie Structurale et Microbiologie, 13402 Marseille Cedex 20, France.
出版信息
J Bacteriol. 1999 Sep;181(17):5505-8. doi: 10.1128/JB.181.17.5505-5508.1999.
Abstract
Hydrogenases, which are ubiquitous in sulfate-reducing bacteria, were previously thought to be absent from Desulfuromonas acetoxidans. For the first time, a hydrogenase from the strict anaerobic sulfur-respiring bacterium D. acetoxidans, grown on ethanol-malate, was detected and enriched. To assay the role of the hydrogenase in the energetic metabolism of D. acetoxidans, we examined the reactivity of the enzyme with polyheme cytochromes from the same bacterium.
摘要
氢化酶在硫酸盐还原菌中普遍存在，以前认为产乙酸脱硫单胞菌中不存在氢化酶。首次检测并富集到了来自严格厌氧的硫呼吸细菌产乙酸脱硫单胞菌的氢化酶，该菌在乙醇-苹果酸培养基上生长。为了分析氢化酶在产乙酸脱硫单胞菌能量代谢中的作用，我们检测了该酶与同一细菌的多血红素细胞色素的反应活性。
相似文献
1
First evidence for the presence of a hydrogenase in the sulfur-reducing bacterium Desulfuromonas acetoxidans.在硫还原细菌氧化醋酸脱硫单胞菌中存在氢化酶的首个证据。
J Bacteriol. 1999 Sep;181(17):5505-8. doi: 10.1128/JB.181.17.5505-5508.1999.
2
Biochemical and spectroscopic characterization of two new cytochromes isolated from Desulfuromonas acetoxidans.从氧化醋酸脱硫单胞菌中分离出的两种新细胞色素的生化与光谱特征
Biochemistry. 1997 Sep 2;36(35):10601-8. doi: 10.1021/bi9707741.
3
Enzymatic reduction of chromate: comparative studies using sulfate-reducing bacteria. Key role of polyheme cytochromes c and hydrogenases.铬酸盐的酶促还原：使用硫酸盐还原菌的比较研究。多血红素细胞色素c和氢化酶的关键作用。
Appl Microbiol Biotechnol. 2001 Jan;55(1):95-100. doi: 10.1007/s002530000467.
4
Multiheme cytochromes from the sulfur-reducing bacterium Desulfuromonas acetoxidans.来自硫还原细菌乙酸氧化脱硫单胞菌的多血红素细胞色素。
Eur J Biochem. 1997 Sep 1;248(2):323-8. doi: 10.1111/j.1432-1033.1997.00323.x.
5
A sequential electron transfer from hydrogenases to cytochromes in sulfate-reducing bacteria.硫酸盐还原菌中氢酶到细胞色素的顺序电子转移。
Biochim Biophys Acta. 2000 Jan 3;1476(1):85-92. doi: 10.1016/s0167-4838(99)00221-6.
6
A quick solution structure determination of the fully oxidized double mutant K9-10A cytochrome c7 from Desulfuromonas acetoxidans and mechanistic implications.来自乙酸氧化脱硫单胞菌的完全氧化双突变体K9-10A细胞色素c7的快速溶液结构测定及其机制意义。
J Biomol NMR. 2002 Feb;22(2):107-22. doi: 10.1023/a:1014202405862.
7
Desulfuromonas acetoxidans gen. nov. and sp. nov., a new anaerobic, sulfur-reducing, acetate-oxidizing bacterium.氧化醋酸脱硫单胞菌属及种，一种新的厌氧、硫还原、醋酸氧化细菌。
Arch Microbiol. 1976 Oct 11;110(1):3-12. doi: 10.1007/BF00416962.
8
The Desulfuromonas acetoxidans triheme cytochrome c7 produced in Desulfovibrio desulfuricans retains its metal reductase activity.在脱硫弧菌中产生的乙酸氧化脱硫单胞菌三血红素细胞色素c7保留了其金属还原酶活性。
Appl Environ Microbiol. 1998 Apr;64(4):1308-12. doi: 10.1128/AEM.64.4.1308-1312.1998.
9
Nuclear-magnetic-resonance studies of Desulfuromonas acetoxidans cytochrome c551.5 (c7).乙酰氧化脱硫单胞菌细胞色素c551.5（c7）的核磁共振研究
Eur J Biochem. 1984 Nov 2;144(3):433-40. doi: 10.1111/j.1432-1033.1984.tb08484.x.
10
Resonance Raman study of multihemic c-type cytochromes from Desulfuromonas acetoxidans.来自氧化脱硫单胞菌的多血红素c型细胞色素的共振拉曼光谱研究。
Eur J Biochem. 2000 Feb;267(4):1050-8. doi: 10.1046/j.1432-1327.2000.01096.x.
引用本文的文献
1
Reconstruction of regulatory and metabolic pathways in metal-reducing delta-proteobacteria.金属还原δ-变形菌中调控和代谢途径的重建
Genome Biol. 2004;5(11):R90. doi: 10.1186/gb-2004-5-11-r90. Epub 2004 Oct 22.
2
A quick solution structure determination of the fully oxidized double mutant K9-10A cytochrome c7 from Desulfuromonas acetoxidans and mechanistic implications.来自乙酸氧化脱硫单胞菌的完全氧化双突变体K9-10A细胞色素c7的快速溶液结构测定及其机制意义。
J Biomol NMR. 2002 Feb;22(2):107-22. doi: 10.1023/a:1014202405862.
本文引用的文献
1
A sequential electron transfer from hydrogenases to cytochromes in sulfate-reducing bacteria.硫酸盐还原菌中氢酶到细胞色素的顺序电子转移。
Biochim Biophys Acta. 2000 Jan 3;1476(1):85-92. doi: 10.1016/s0167-4838(99)00221-6.
2
3
Structural and kinetic studies of the Y73E mutant of octaheme cytochrome c3 (Mr = 26 000) from Desulfovibrio desulfuricans Norway.来自挪威脱硫弧菌的八聚体细胞色素c3（Mr = 26000）Y73E突变体的结构和动力学研究
Biochemistry. 1998 Feb 24;37(8):2120-30. doi: 10.1021/bi971656g.
4
Genetic diversity and expression of the [NiFe] hydrogenase large-subunit gene of Desulfovibrio spp. in environmental samples.脱硫弧菌属环境样品中[NiFe]氢化酶大亚基基因的遗传多样性与表达
Appl Environ Microbiol. 1997 Nov;63(11):4360-9. doi: 10.1128/aem.63.11.4360-4369.1997.
5
Multiheme cytochromes from the sulfur-reducing bacterium Desulfuromonas acetoxidans.来自硫还原细菌乙酸氧化脱硫单胞菌的多血红素细胞色素。
Eur J Biochem. 1997 Sep 1;248(2):323-8. doi: 10.1111/j.1432-1033.1997.00323.x.
6
Functional and structural role of the cytochrome b subunit of the membrane-bound hydrogenase complex of Alcaligenes eutrophus H16.嗜碱假单胞菌H16膜结合氢化酶复合体细胞色素b亚基的功能和结构作用
Eur J Biochem. 1997 Aug 15;248(1):179-86. doi: 10.1111/j.1432-1033.1997.00179.x.
7
Biochemical and spectroscopic characterization of two new cytochromes isolated from Desulfuromonas acetoxidans.从氧化醋酸脱硫单胞菌中分离出的两种新细胞色素的生化与光谱特征
Biochemistry. 1997 Sep 2;36(35):10601-8. doi: 10.1021/bi9707741.
8
NMR characterization and solution structure determination of the oxidized cytochrome c7 from Desulfuromonas acetoxidans.来自乙酸脱硫单胞菌的氧化型细胞色素c7的核磁共振表征及溶液结构测定
Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14396-400. doi: 10.1073/pnas.93.25.14396.
9
Purification and characterization of a membrane-bound hydrogenase from Sporomusa sphaeroides involved in energy-transducing electron transport.来自球形芽孢八叠球菌的参与能量转换电子传递的膜结合氢化酶的纯化与特性分析
Arch Microbiol. 1996 Feb;165(2):141-7. doi: 10.1007/s002030050309.
10
Voltammetric studies of the catalytic electron-transfer process between the Desulfovibrio gigas hydrogenase and small proteins isolated from the same genus.巨大脱硫弧菌氢化酶与从同一属中分离出的小蛋白质之间催化电子转移过程的伏安法研究。
Eur J Biochem. 1993 Nov 1;217(3):981-9. doi: 10.1111/j.1432-1033.1993.tb18329.x.
Zotero 插件