Goldring J D, Padayachee T, Ismail I
School of Molecular and Cellular Biosciences, University of Natal, South Africa.
Mem Inst Oswaldo Cruz. 1999 Sep-Oct;94(5):667-74. doi: 10.1590/s0074-02761999000500021.
An assay was developed measuring the disruption of rosettes between Plasmodium falciparuminfected (trophozoites) and uninfected erythrocytes by the antimalarial drugs quinine, artemisinin mefloquine, primaquine, pyrimethamine, chloroquine and proguanil. At 4 hr incubation rosettes were disrupted by all the drugs in a dose dependent manner. Artemisinin and quinine were the most effective anti-malarials at disrupting rosettes at their therapeutic concentrations with South African RSA 14, 15, 17 and The Gambian FCR-3 P. falciparum strains. The least effective drugs were proguanil and chloroquine. A combination of artemisinin and mefloquine was more effective than each drug alone. The combinations of pyrimethamine or primaquine, with quinine disrupted more rosettes than quinine alone. Quinine may be an effective drug in the treatment of severe malaria because the drug efficiently reduces the number of rosettes.
开发了一种检测方法，用于测量抗疟药物奎宁、青蒿素、甲氟喹、伯氨喹、乙胺嘧啶、氯喹和氯胍对恶性疟原虫感染的（滋养体）与未感染红细胞之间玫瑰花结的破坏作用。在孵育4小时时，所有药物均以剂量依赖性方式破坏玫瑰花结。青蒿素和奎宁在其治疗浓度下对破坏南非RSA 14、15、17和冈比亚FCR-3恶性疟原虫菌株的玫瑰花结是最有效的抗疟药物。效果最差的药物是氯胍和氯喹。青蒿素和甲氟喹的组合比单独使用每种药物更有效。乙胺嘧啶或伯氨喹与奎宁的组合比单独使用奎宁破坏更多的玫瑰花结。奎宁可能是治疗重症疟疾的有效药物，因为该药物能有效减少玫瑰花结的数量。
