老禽直肠盲肠制剂用于研究β-肾上腺素能药物选择性的适用性。

Suitability of the old fowl rectal caecum preparation for investigating the selectivity of beta-adrenergic drugs.

作者信息

Carcano R, Belloli C, Arioli F, Beretta C

机构信息

Institute of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Milan, Italy.

出版信息

J Pharmacol Toxicol Methods. 1998 Nov;40(4):221-5. doi: 10.1016/s1056-8719(99)00009-x.

DOI:10.1016/s1056-8719(99)00009-x

PMID:10465157

Abstract

We tested alpha- and beta-adrenergic drugs on isolated strips of fowl rectal caecum from 14- to 16-week-old Warren hens. Basal tone and spontaneous motility were dose-dependently reduced by isoprenaline and all the selective beta-agonists tested (except xamoterol) with the following order of potency: isoprenaline=fenoterol=procaterol=clenbuterol>dobutamine> SR58611A. The results indicate that this tissue preparation consists almost entirely of beta2-adrenoceptors. This preparation may, therefore, be considered a suitable assay for discriminating beta1- from beta2-agonists according to their selectivity.

摘要

我们对14至16周龄的沃伦母鸡分离出的家禽直肠盲肠条进行了α和β肾上腺素能药物测试。异丙肾上腺素以及所有测试的选择性β激动剂（除了xamoterol）均能剂量依赖性地降低基础张力和自发运动性，其效力顺序如下：异丙肾上腺素=非诺特罗=丙卡特罗=克仑特罗>多巴酚丁胺>SR58611A。结果表明，该组织制剂几乎完全由β2肾上腺素能受体组成。因此，该制剂可被视为一种根据β1和β2激动剂的选择性来区分它们的合适检测方法。