Conroy D M, Sirois P
Institute of Pharmacology of Sherbrooke, Faculty of Medicine, University of Sherbrooke, PQ, Canada.
Inflammation. 1999 Oct;23(5):437-48. doi: 10.1023/a:1021913009742.
The effects of an intravenous injection (i.v.) of Sephadex beads (20 mg kg(-1)) were examined on bronchial responsiveness to ACh (1-200 microg kg(-1) i.v.) as well as on cell accumulation in guinea-pig lung. Bronchial hyperreactivity to ACh, measured as increase in pulmonary insufflation pressure (PIP), was observed 3 h following the i.v. injection of Sephadex beads. However, no significant increase in bronchial reactivity to ACh was measured at 6 and 12 h following Sephadex injection. A second later increase in bronchial hyperresponsiveness was observed at 24 h. Bronchoalveolar lavage performed at 3 h following Sephadex treatment showed that there was no significant increase in total or differential cell number. At 6 h and 12 h, a significant increase in total cell counts was observed. At 24 h, a greater than 5-fold increase in cell number was observed and was related to a marked eosinophil, neutrophil and macrophage infiltration. A platelet-activating factor (PAF) antagonist, CV-3988 (10 mg kg(-1) i.v.), and a thromboxane A2 (TxA2) antagonist, L655,240 (10 mg kg(-1) i.v.), significantly attenuated the Sephadex-induced bronchial hyperresponsiveness to ACh observed at 3 h. The results show that an i.v. injection of Sephadex beads in guinea pigs can induce an early bronchial hyperresponsiveness to ACh that is mediated by the release of both PAF and TxA2 and is independent of airway cell infiltration.
研究了静脉注射（i.v.）葡聚糖珠（20 mg kg(-1)）对豚鼠肺支气管对乙酰胆碱（ACh，1 - 200 μg kg(-1) i.v.）的反应性以及细胞积聚的影响。静脉注射葡聚糖珠3小时后，观察到支气管对ACh的高反应性，以肺通气压力（PIP）增加来衡量。然而，在注射葡聚糖珠后的6小时和12小时，未检测到支气管对ACh反应性的显著增加。在24小时观察到支气管高反应性再次增加。葡聚糖处理后3小时进行的支气管肺泡灌洗显示，总细胞数或细胞分类计数均无显著增加。在6小时和12小时，观察到总细胞计数显著增加。在24小时，观察到细胞数增加超过5倍，且与明显的嗜酸性粒细胞、中性粒细胞和巨噬细胞浸润有关。血小板活化因子（PAF）拮抗剂CV - 3988（10 mg kg(-1) i.v.）和血栓素A2（TxA2）拮抗剂L655,240（10 mg kg(-1) i.v.）显著减轻了在3小时观察到的葡聚糖诱导的支气管对ACh的高反应性。结果表明，豚鼠静脉注射葡聚糖珠可诱导早期支气管对ACh的高反应性，这是由PAF和TxA2的释放介导的，且与气道细胞浸润无关。
