一种构建E1和E1/E4缺失型重组腺病毒载体的简单方法。
A simple method for constructing E1- and E1/E4-deleted recombinant adenoviral vectors.
作者信息
Mizuguchi H, Kay M A
机构信息
Division of Biological Chemistry and Biologicals, National Institute of Health Sciences, Tokyo, Japan.
出版信息
Hum Gene Ther. 1999 Aug 10;10(12):2013-7. doi: 10.1089/10430349950017374.
PMID:10466635
Abstract
We previously developed a two-plasmid in vitro ligation method that did not require a recombination step to produce new recombinant E1- or E1/E3-deleted adenoviral vectors. In this study, we have modified the system to improve the simplicity of vector construction and, in addition, to allow for production of an E1/E4-deleted vector.
摘要
我们之前开发了一种双质粒体外连接方法，该方法无需重组步骤即可产生新的缺失E1或E1/E3的重组腺病毒载体。在本研究中，我们对该系统进行了改进，以提高载体构建的简便性，此外，还可用于生产缺失E1/E4的载体。
Zotero 插件