Attallah A M, Yones E, Ismail H, El Masry S A, Tabll A, Elenein A A, El Ghawalby N A
Biotechnology Research Center, New Damietta City, Egypt.
Am J Trop Med Hyg. 1999 Mar;60(3):493-7. doi: 10.4269/ajtmh.1999.60.493.
Schistosoma circulating antigens were used for the detection of active infection. Anti-S. mansoni IgG2a monoclonal antibody (MAb) designated C5C4 was generated. The target epitope of this MAb was detected in adult worms, eggs, and cercariae antigenic extracts of S. mansoni and S. haematobium, had a molecular size of 63 kD, and was not detected in Fasciola hepatica and Ascaris. In addition, a 50-kD degradation product was identified only in the urine of infected individuals. Analysis by high-performance liquid chromatography of the purified antigen demonstrated only one peak. The 63-kD antigen was characterized as a protein containing 40.4% hydrophobic, 7.5% acidic, and 8.8% basic amino acids. The C5C4 MAb was used in a Fast Dot-ELISA for rapid and simple diagnosis of human schistosomiasis. The 63-kD circulating antigen was detected in 92% of urine samples from 330 S. mansoni-infected individuals, with 16% false-positive results among 130 noninfected individuals.
血吸虫循环抗原用于检测活动性感染。制备了一种抗曼氏血吸虫IgG2a单克隆抗体（MAb），命名为C5C4。该单克隆抗体的靶表位在曼氏血吸虫和埃及血吸虫的成虫、虫卵及尾蚴抗原提取物中被检测到，分子大小为63 kD，在肝片吸虫和蛔虫中未检测到。此外，仅在感染个体的尿液中鉴定出一种50-kD的降解产物。对纯化抗原进行高效液相色谱分析仅显示一个峰。63-kD抗原的特征为一种蛋白质，含40.4%的疏水氨基酸、7.5%的酸性氨基酸和8.8%的碱性氨基酸。C5C4单克隆抗体用于快速斑点酶联免疫吸附测定（Fast Dot-ELISA），以快速、简便地诊断人体血吸虫病。在330例曼氏血吸虫感染个体的尿液样本中，92%检测到63-kD循环抗原，在130例未感染个体中有16%出现假阳性结果。
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