Attallah A M, El Masry S A, Ismail H, Attia H, Abdel Aziz M, Shehatta A S, Tabll A, Soltan A, El Wassif A
Biotechnology Research Center, New Damietta City, Egypt.
J Parasitol. 1998 Apr;84(2):301-6.
A polypeptide antigen of 74.0 kDa molecular weight was detected in the antigenic extracts of the 3 developmental stages of Schistosoma mansoni (eggs, cercariae, and adult worms) by western blotting using BRL4 monoclonal antibody (mAb) that significantly protected mice at the levels of 51.6%, 42%, and 53.8% against challenge S. mansoni infection in 3 separate experiments. This antigen was isolated and purified from crude soluble worm antigen preparation by immunoaffinity chromatography using CNBr-activated sepharose-4B beads coupled with the BRL4 mAb. The purified antigen showed a single peak when analyzed by both high-performance liquid chromatography and high-performance capillary electrophoresis. The 74-kDa antigen was characterized as a protein in nature with 56.9% hydrophilic amino acids and 43.1% hydrophobic amino acids. This antigen was detected in 93% of urine samples from infected cases with specificity of 89% among noninfected cases using an enzyme immunoassay-fast dot-enzyme-linked immunosorbent assay based on BRL4 mAb.
使用BRL4单克隆抗体（mAb）通过蛋白质印迹法在曼氏血吸虫的3个发育阶段（虫卵、尾蚴和成虫）的抗原提取物中检测到一种分子量为74.0 kDa的多肽抗原，在3个独立实验中，该抗原分别以51.6%、42%和53.8%的水平显著保护小鼠免受曼氏血吸虫感染的攻击。通过使用与BRL4 mAb偶联的溴化氰活化的琼脂糖-4B珠进行免疫亲和层析，从粗可溶性虫体抗原制剂中分离并纯化了该抗原。通过高效液相色谱和高效毛细管电泳分析时，纯化的抗原显示出单一峰。该74-kDa抗原本质上被鉴定为一种蛋白质，其中亲水氨基酸占56.9%，疏水氨基酸占43.1%。使用基于BRL4 mAb的酶免疫测定-快速斑点酶联免疫吸附测定法，在93%的感染病例尿液样本中检测到该抗原，在未感染病例中的特异性为89%。
