Aslankaraoglu E, Gürhan S I, Gümüşderelioglu M
Chemical Engineering Department, Hacettepe University, 06532 Beytepe, Ankara, Turkey.
Biotechnol Appl Biochem. 1999 Aug;30(1):65-71.
Two types of baby-hamster kidney (BHK) cell line, anchorage-dependent BHK-An(32) and suspended BHK-An(48) cells, were cultivated on non-woven polyester fabrics (NWPF) that were prepared from non-woven polyethylene teraphthalate sheets with different void fractions in three-dimensional configuration. The attachment and growth characteristics of both cell lines were investigated in stationary and submerged cultures, containing Dulbecco's modified Eagle's medium supplemented with fetal-calf serum. All cultures displayed rapid attachment (<120 min) of inoculated cells to the discs. The growth of BHK cells on NWPF discs in stationary cultures was similar to that observed in tissue-culture dishes; however, the denser discs showed better results in terms of final cell density, especially in the case of BHK-An(48) cells. Spinner-flask cultures indicated that higher cell yields were obtained than in stationary conditions. Microscopic observation showed that anchorage-dependent BHK-An(32) cells spread through the interfibre spaces of NPWF discs to form three-dimensional structures whereas BHK-An(48) cells proliferated on fibres.
两种类型的幼仓鼠肾（BHK）细胞系，即贴壁依赖性的BHK - An(32)细胞和悬浮的BHK - An(48)细胞，在由具有不同孔隙率的三维构型的非织造聚对苯二甲酸乙二酯片材制备的非织造聚酯织物（NWPF）上进行培养。在含有添加胎牛血清的杜尔贝科改良伊格尔培养基的静置培养和浸没培养中，研究了这两种细胞系的附着和生长特性。所有培养物中接种的细胞均在120分钟内快速附着于圆盘。在静置培养中，BHK细胞在NWPF圆盘上的生长与在组织培养皿中观察到的相似；然而，密度较大的圆盘在最终细胞密度方面显示出更好的结果，特别是对于BHK - An(48)细胞。旋转瓶培养表明，与静置条件相比可获得更高的细胞产量。显微镜观察显示，贴壁依赖性的BHK - An(32)细胞通过NPWF圆盘的纤维间空间扩散形成三维结构，而BHK - An(48)细胞则在纤维上增殖。
