Kahler E, Waller C, Rommel E, Belle V, Hiller K H, Voll S, Bauer W R, Haase A
Physikalisches Institut, Universität Würzburg, Würzburg, Germany.
Magn Reson Med. 1999 Sep;42(3):500-6. doi: 10.1002/(sici)1522-2594(199909)42:3<500::aid-mrm12>3.0.co;2-3.
Measurement of regional blood volume (RBV) in the myocardium in vivo is important for the assessment of tissue viability and function. The method in this work is based on the acquisition of a T(1) map before and after intravascular contrast agent application. It is known that this method is influenced by perfusion that causes an overestimation of RBV values. In order to solve this problem, the new method is proposed which acquires T(1) maps with slice selective inversion pulses. Due to blood flow nonexcited spins enter the detection slice, which leads to an acceleration of the relaxation time. A model that divides tissue into two compartments is adapted to slice selective inversion in order to derive a simple expression for perfusion-corrected RBV. The aim of the study is to demonstrate the feasibility and accuracy of this technique for quantification of RBV in rat myocardium in vivo. RBV maps were obtained for five rats, and the reproducibility was determined by repeating the experiment several times. A mean RBV value of 12.8 +/- 0.7% (v/v) over all animals was obtained in the myocardium. The results were compared with RBV maps obtained with perfusion-sensitive RBV imaging in the same five rats and with first-pass RBV studies. In order to demonstrate the strength of the new method the vasodilator adenosine was administered and alterations in microcirculation were imaged. Magn Reson Med 42:500-506, 1999.
体内测量心肌局部血容量（RBV）对于评估组织活力和功能很重要。本研究中的方法基于在血管内注射造影剂前后采集T(1)图谱。已知该方法受灌注影响，会导致RBV值高估。为解决此问题，提出了一种新方法，即利用层面选择反转脉冲采集T(1)图谱。由于血流，未被激发的自旋进入检测层面，导致弛豫时间加快。将组织分为两个腔室的模型适用于层面选择反转，以便推导出血流校正后RBV的简单表达式。本研究的目的是证明该技术在体内定量大鼠心肌RBV的可行性和准确性。对五只大鼠获取了RBV图谱，并通过多次重复实验确定了其可重复性。所有动物心肌的平均RBV值为12.8±0.7%（体积/体积）。将结果与在同五只大鼠中通过灌注敏感型RBV成像和首过RBV研究获得的RBV图谱进行了比较。为证明新方法的优势，给予血管扩张剂腺苷并对微循环变化进行成像。《磁共振医学》42:500 - 506, 1999年。
