Fischmeister G, Zoubek A, Jugovic D, Witt V, Ladenstein R, Fritsch G, Höcker P, Gadner H, Kovar H
St Anna Children's Hospital and Children's Cancer Research Institute, Vienna, Austria.
Bone Marrow Transplant. 1999 Aug;24(4):405-9. doi: 10.1038/sj.bmt.1701924.
Reverse transcriptase polymerase chain reaction (RT-PCR) was applied to evaluate the frequency of tumour cells in PBPC products from 15 high risk Ewing tumour (ET) patients who were treated according to EICESS 92 with high-dose chemotherapy (HDC) and stem cell rescue. Initial tumour cell contamination of the bone marrow (BM) detected by light microscopy was found in five and by RT-PCR in eight cases. RT-PCR was performed on each PBPC sample repeatedly at a sensitivity comparable to 20-100 highly EWS-Fli1 expressing tumour cells per 10 ml of fresh blood. Irrespective of the extent of BM involvement at diagnosis, all BM samples obtained before harvest were RT-PCR negative. Among 12 of 35 analysed apheresis products with single positive RT-PCR results only one sample tested reproducibly positive for tumour cell contamination in independent determinations. These preliminary data suggest that tumour cell contamination of PBPC is rarely found in patients with ET.
逆转录酶聚合酶链反应（RT-PCR）用于评估15例高危尤因肉瘤（ET）患者外周血造血干细胞（PBPC）产品中肿瘤细胞的频率，这些患者按照EICESS 92方案接受了大剂量化疗（HDC）和干细胞救援。通过光学显微镜检测，发现5例患者骨髓（BM）存在初始肿瘤细胞污染，通过RT-PCR检测则发现8例。对每个PBPC样本反复进行RT-PCR，其灵敏度相当于每10毫升新鲜血液中有20 - 100个高表达EWS-Fli1的肿瘤细胞。无论诊断时BM受累程度如何，采集前获得的所有BM样本RT-PCR均为阴性。在35份分析的单采血液成分产品中，12份RT-PCR结果呈单阳性，其中只有1份样本在独立检测中可重复检测出肿瘤细胞污染呈阳性。这些初步数据表明，ET患者中很少发现PBPC存在肿瘤细胞污染。
