Neubauer H, Reischl U, Köstler J, Aleksic S, Finke E J, Meyer H
Institute of Microbiology, German Armed Forces Medical Academy, München, Germany.
Zentralbl Bakteriol. 1999 Jul;289(3):329-37. doi: 10.1016/s0934-8840(99)80070-x.
Four identification systems were used to type Yersinia enterocolitica strain Y11 and Yersinia enterocolitica sensu strictoT. Two systems based on biochemical reaction patterns identified both strains as Yersinia enterocolitica. Two molecular assays targeting the 16S rRNA gene failed to identify either strain Y11 or the type strain. Therefore, both strains were typed by the classical taxonomical approach requiring a determination of the overall base composition and the base sequence similarity using hybridization. Again both strains were classified as Yersinia enterocolitica isolates. Consequently, the sequences of the 16S rRNA gene of both strains were determined and compared. The strains differed in a region where nucleotide changes between species of the genus Yersinia had been described earlier. These differences may explain the failure of the molecular assays to identify the strains. They also demonstrate an independent evolution of the 16S rRNA genes in the species Yersinia enterocolitica sensu stricto suggesting an amendment to the nomenclature to be used in the future.
使用了四种鉴定系统对小肠结肠炎耶尔森氏菌菌株Y11和狭义小肠结肠炎耶尔森氏菌进行分型。两种基于生化反应模式的系统将这两种菌株均鉴定为小肠结肠炎耶尔森氏菌。两种针对16S rRNA基因的分子检测方法均未能鉴定出菌株Y11或模式菌株。因此，采用经典分类学方法对这两种菌株进行分型，该方法需要通过杂交来确定总体碱基组成和碱基序列相似性。同样，这两种菌株均被归类为小肠结肠炎耶尔森氏菌分离株。因此，测定并比较了这两种菌株16S rRNA基因的序列。这两种菌株在一个区域存在差异，此前已描述过耶尔森氏菌属不同种之间的核苷酸变化。这些差异可能解释了分子检测方法无法鉴定这些菌株的原因。它们还表明狭义小肠结肠炎耶尔森氏菌中16S rRNA基因的独立进化，这表明未来可能需要对命名法进行修订。
