Nijman I J, Bradley D G, Hanotte O, Otsen M, Lenstra J A
Department of Bacteriology, Faculty of Veterinary Medicine, Utrecht, The Netherlands.
Anim Genet. 1999 Aug;30(4):265-73. doi: 10.1046/j.1365-2052.1999.00475.x.
We describe satellite DNA variation that detects hybridization of Bos indicus (zebu or indicine cattle) and Bos taurus (taurine cattle) in African cattle populations. On Southern blots hybridized to a satellite III probe, relative intensities of Hinfl fragments correlated with the taurine-zebu composition in hybrid animals as deduced from AFLP genotyping of the same animals and previous data on microsatellite allele frequencies. Similar results were obtained by PCR-RFLP analysis of a zebu-specific mutation in the repeat unit of satellite 1.711b. Analysis of individuals from 20 African cattle breeds indicate that the centromeric satellites of the sanga breeds are of the taurine type and that several East-African zebu breeds are hybrids between taurine and zebu. These satellite RFLP, or SFLP, markers provide a fast method to screen the genetic makeup of African cattle.
我们描述了卫星DNA变异情况，该变异可检测非洲牛种群中瘤牛（泽布牛或印度牛）和普通牛（黄牛）的杂交情况。在与卫星III探针杂交的Southern杂交印迹上，Hinfl片段的相对强度与杂交动物中的黄牛-瘤牛组成相关，这是根据对同一动物的AFLP基因分型以及先前关于微卫星等位基因频率的数据推断得出的。通过对卫星1.711b重复单元中的一个瘤牛特异性突变进行PCR-RFLP分析，也获得了类似的结果。对20个非洲牛品种的个体进行分析表明，桑加品种的着丝粒卫星属于黄牛类型，并且几个东非瘤牛品种是黄牛和瘤牛的杂交品种。这些卫星RFLP或SFLP标记提供了一种快速筛选非洲牛遗传组成的方法。
