膀胱移行细胞癌的图像细胞术DNA分析及增殖细胞核抗原（PCNA）表达

Image cytometric DNA analysis and proliferating cell nuclear antigen (PCNA) expression in transitional cell carcinoma of the bladder.

作者信息

Ioakim-Liossi A G, Karakitsos P J, Pantazopoulos D, Aroni K G, Athanossiadou P

机构信息

Department of Pathology, Medical University of Athens, Greece.

出版信息

Cancer Detect Prev. 1999;23(5):401-7. doi: 10.1046/j.1525-1500.1999.99048.x.

DOI:10.1046/j.1525-1500.1999.99048.x

PMID:10468892

Abstract

The proliferation rate as determined by Proliferating Cell Nuclear Antigen (PCNA) immunostaining and the DNA ploidy status as measured by static cytometry were studied in 70 transitional cell carcinomas of the bladder (TCCB) in relation to grade, stage, and recurrence. The follow-up period was 2 years. A significant difference was observed in PCNA expression among grades I, II, and III (P < 0.02), between superficial (pTa-pT1) and invasive (pT2-pT4) tumors (p < 0. 04), between recurring and non-recurring tumors (p < 0.001), and between tumors of the same grade with and without recurrence (p < 0. 05). A significant difference was also found in the ploidy pattern among grades I, II, and III (p = 0.002), and between superficial and invasive (p = 0.02) and recurring and non-recurring tumors (p < 0. 01). Finally, the recurrence status seems to be strongly influenced by the proliferation rate and ploidy of TCCB (p < 0.001). Our results suggest that the above-studied parameters may offer useful information on the biological behavior of TCCB.

摘要

通过增殖细胞核抗原（PCNA）免疫染色测定的增殖率以及通过静态细胞计数法测量的DNA倍体状态，在70例膀胱移行细胞癌（TCCB）中进行了研究，研究内容涉及肿瘤的分级、分期和复发情况。随访期为2年。I级、II级和III级之间的PCNA表达存在显著差异（P < 0.02），浅表性（pTa - pT1）肿瘤与浸润性（pT2 - pT4）肿瘤之间存在显著差异（p < 0.04），复发肿瘤与未复发肿瘤之间存在显著差异（p < 0.001），同一分级的复发肿瘤与未复发肿瘤之间也存在显著差异（p < 0.05）。I级、II级和III级之间的倍体模式也存在显著差异（p = 0.002），浅表性肿瘤与浸润性肿瘤之间（p = 0.02）以及复发肿瘤与未复发肿瘤之间（p < 0.01）也存在显著差异。最后，复发状态似乎受TCCB的增殖率和倍体的强烈影响（p < 0.001）。我们的结果表明，上述研究参数可能为TCCB的生物学行为提供有用信息。