Simons A, Schepens M, Forus A, Godager L, van Asseldonk M, Myklebost O, van Kessel A G
Department of Human Genetics, University Hospital, Nijmegen, The Netherlands.
Genes Chromosomes Cancer. 1999 Oct;26(2):115-24.
Representational difference analysis (RDA) of a human osteosarcoma xenograft resulted in the isolation of four tumor-associated homozygously deleted DNA fragments, all originating from chromosome 4, region q32-q34. Southern blot analysis using the RDA fragments and interphase FISH analysis using PACs corresponding to these RDA fragments revealed allelic loss of the 4q32-q34 region in 17 of 27 (63%) osteosarcomas tested. These results suggest the involvement of tumor suppressor gene(s) within this chromosomal region in osteosarcoma development. The RDA fragments and corresponding PAC clones will be instrumental in the isolation of such gene(s). Genes Chromosomes Cancer 26:115-124, 1999.
对人骨肉瘤异种移植瘤进行代表性差异分析（RDA），结果分离出4个与肿瘤相关的纯合缺失DNA片段，均来自4号染色体q32 - q34区域。使用这些RDA片段进行Southern印迹分析，以及使用与这些RDA片段对应的PACS进行间期荧光原位杂交分析，结果显示在27个受试骨肉瘤中有17个（63%）存在4q32 - q34区域的等位基因缺失。这些结果提示该染色体区域内的肿瘤抑制基因（一个或多个）参与了骨肉瘤的发生发展。这些RDA片段和相应的PACS克隆将有助于分离此类基因。《基因、染色体与癌症》26:115 - 124, 1999年。
