内皮标志物CD 34在卵巢癌中的预后相关性
Prognostic relevance of the endothelial marker CD 34 in ovarian cancer.
作者信息
Heimburg S, Oehler M K, Papadopoulos T, Caffier H, Kristen P, Dietl J
机构信息
Department of Obstetrics and Gynaecology, University of Würzburg, Germany.
出版信息
Anticancer Res. 1999 Jul-Aug;19(4A):2527-9.
BACKGROUND
Tumour angiogenesis and microvessel density are of prognostic significance in several human neoplasia. To investigate how tumour vascularity correlates with disease-free survival microvessel density was assessed in 38 patients with ovarian cancer using the highly specific endothelial marker CD 34.
METHODS
Representative specimens were obtained and stained using monoclonal CD 34 antibodies. The microvessels were quantified at 200x and 400x magnification in the most active areas of neovascularisation. Degree of angiogenesis was correlated with histologic tumour type, grading, and tumour stage. Furthermore, survival was calculated using Kaplan-Meier analysis and results again correlated with the microvessel count.
RESULTS
No correlation was found between microvessel density, histologic type, grading, and FIGO stage. Patients with a vessel count more than 40 (200x magnification) had a statistically significant lower overall survival (p = 0.022).
CONCLUSION
Our results indicate that CD 34 is a useful marker in determining tumour neovascularisation which might be of prognostic relevance in patients with ovarian cancer.
背景
肿瘤血管生成和微血管密度在多种人类肿瘤中具有预后意义。为了研究肿瘤血管生成与无病生存期的相关性，我们使用高度特异性的内皮标志物CD 34对38例卵巢癌患者的微血管密度进行了评估。
方法
获取代表性标本，并用抗CD 34单克隆抗体进行染色。在新生血管形成最活跃的区域，于200倍和400倍放大倍数下对微血管进行定量。血管生成程度与组织学肿瘤类型、分级及肿瘤分期相关。此外，采用Kaplan-Meier分析计算生存率，并将结果再次与微血管计数相关联。
结果
微血管密度与组织学类型、分级及国际妇产科联盟（FIGO）分期之间未发现相关性。微血管计数超过40（200倍放大倍数）的患者总生存期在统计学上显著较低（p = 0.022）。
结论
我们的结果表明，CD 34是确定肿瘤新生血管生成的有用标志物，这可能对卵巢癌患者的预后具有相关性。
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