Grover M, Gaur T, Kochhar A, Maheshwari S C, Tyagi A K
Department of Plant Molecular Biology, University of Delhi South Campus, India.
DNA Res. 1999 Jun 30;6(3):173-7. doi: 10.1093/dnares/6.3.173.
The psbQ gene encoding a 16-kDa polypeptide of the oxygen-evolving complex of photosystem II has been isolated from Arabidopsis thaliana and characterized. The gene consists of a 28 nucleotide long leader sequence, two introns and three exons encoding a 223-amino-acid precursor polypeptide. The first 75 amino acids act as a transit peptide for the translocation of the polypeptide into the thylakoid lumen. Expression studies show that the gene is light-inducible and expresses only in green tissues with high steady-state mRNA levels in leaves. Using this gene as a probe, restriction fragment length polymorphism between two ecotypes, Columbia and Estland, has also been detected.
