Skarda J
Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Prague.
Physiol Res. 1999;48(1):43-9.
Young castrated male goats (n = 8) were used to investigate the effect of long-term treatment with recombinant methionyl bovine somatotropin in a sustained release vehicle (bST; 100 mg at seven-day intervals in a 147-day experiment) and chronic culture (24 h) of omental adipose tissue in the presence of various hormones on lipogenic responses to catecholamines during acute incubation (2 h) in a sodium acetate supplemented glucose-free buffer. The rate of fatty acid synthesis in freshly-prepared adipose explants was low and did not differ from those cultured in the absence of hormones for 24 h. Hormonal combination of insulin (17 nmol.l(-1)) plus cortisol (138 nmol.l(-1)) or insulin plus recombinant enterokinase linker bST (4.5 nmol.l(-1) increased lipogenesis (P<0.05). Further addition of bST or cortisol decreased lipogenesis significantly (P<0.05) in the controls but not significantly in bST-treated animals. Cultured explants from either control or bST-treated animals showed significant inhibition of lipogenesis by both norepinephrine (10 micromol.l(-1)) and isoprenaline (10 micromol.l(-1)). BST treatment in vivo did not increase the responsiveness of cultured explants to norepinephrine in vitro, however, the responsiveness to isoprenaline(inhibition of lipogenesis) was greater in bST-treated animals than in the controls.
选用8只年轻去势雄山羊，研究在持续释放载体中用重组蛋氨酰牛生长激素（bST；在为期147天的实验中，每隔7天注射100毫克）长期治疗以及在添加醋酸钠的无葡萄糖缓冲液中急性孵育（2小时）期间，网膜脂肪组织在各种激素存在下进行慢性培养（24小时）对儿茶酚胺脂肪生成反应的影响。新鲜制备的脂肪外植体中脂肪酸合成速率较低，与在无激素条件下培养24小时的外植体没有差异。胰岛素（17 nmol·l⁻¹）加皮质醇（138 nmol·l⁻¹）或胰岛素加重组肠激酶连接bST（4.5 nmol·l⁻¹）的激素组合增加了脂肪生成（P<0.05）。进一步添加bST或皮质醇在对照组中显著降低了脂肪生成（P<0.05），但在接受bST治疗的动物中没有显著降低。来自对照组或接受bST治疗动物的培养外植体均显示去甲肾上腺素（10 μmol·l⁻¹）和异丙肾上腺素（10 μmol·l⁻¹）对脂肪生成有显著抑制作用。然而，体内bST治疗并未增加培养外植体对体外去甲肾上腺素的反应性，不过，接受bST治疗的动物对异丙肾上腺素（脂肪生成抑制）的反应性比对照组更高。
