Iwata T, Umezawa K, Toyoda F, Takahashi N, Matsukawa H, Yamamoto K, Miura S, Hayashi H, Kikuyama S
Department of Biology, School of Education, Waseda University, Nishi-waseda 1-6-1, Shinjuku-ku, Tokyo, Japan.
FEBS Lett. 1999 Sep 3;457(3):400-4. doi: 10.1016/s0014-5793(99)01062-5.
Cloning of cDNA encoding a decapeptide pheromone (sodefrin) that attracts conspecific female newts was attempted. A cDNA clone encoding a protein consisting of 189 amino acid residues including a sodefrin sequence was isolated from a Cynops pyrrhogaster abdominal gland cDNA library. Likewise, a cDNA clone encoding a molecule comparable to the sodefrin precursor was obtained from a Cynops ensicauda abdominal gland cDNA library. This clone encoded a precursor protein of 192 amino acid residues, including a sodefrin-like peptide sequence with substitutions of two amino acid residues. This is the first report of a peptide pheromone precursor in vertebrates.
尝试克隆编码一种能吸引同种雌性蝾螈的十肽信息素（精包素）的cDNA。从日本红腹蝾螈腹部腺体cDNA文库中分离出一个编码由189个氨基酸残基组成的蛋白质（包括精包素序列）的cDNA克隆。同样，从琉球棘螈腹部腺体cDNA文库中获得了一个编码与精包素前体类似分子的cDNA克隆。该克隆编码一个由192个氨基酸残基组成的前体蛋白，包括一个有两个氨基酸残基替换的类精包素肽序列。这是脊椎动物中肽类信息素前体的首次报道。
