Oztürk K, Durusoy M
Department of Molecular Biology, Faculty of Science, Hacettepe University, Ankara, Turkey.
Toxicol Lett. 1999 Jul 30;108(1):63-8. doi: 10.1016/s0378-4274(99)00117-4.
Four nitroarenes were tested in two standard genotoxicity assay systems using three well-known bacterial tester strains. The results were as follows: 4-nitroquinoline l-oxide (4NQO) was positive in Quillardet and Hofnung's SOS chromotest using Escherichia coli strain PQ37 both in the presence and absence of microsomal (S9) supplements and in the Salmonella typhimurium umu tester strains NM2009 and NM3009, which express high levels of O-acetyltransferase (O-AT) and O-AT plus nitroreductase (NR) respectively. m-Nitrocinnamic acid (m-NCA) was weakly positive in strains NM2009 and NM3009, but negative in the SOS chromotest; m-dinitrobenzene (m-DNB) was weakly positive in strain NM2009, intermediate positive in strain NM3009, but negative in the SOS chromotest; 2,4-dinitrotoluene (2,4-DNT) was weakly positive in strain NM3009, but negative in strain NM2009 and in the SOS chromotest. However it still showed a dose-response relationship in strain NM2009. In view of these results, it is suggested that investigators planning to screen miscellaneous nitroarenes for their genotoxicity in the future should consider taking advantage of the increased sensitivity which is conferred on S. typhimurium strains NM2009 and NM3009 by virtue of their capacity to overexpress O-AT or O-AT and NR.
使用三种著名的细菌测试菌株，在两个标准遗传毒性检测系统中对四种硝基芳烃进行了测试。结果如下：4-硝基喹啉-1-氧化物（4NQO）在使用大肠杆菌菌株PQ37的Quillardet和Hofnung的SOS显色测试中呈阳性，无论有无微粒体（S9）补充剂，在鼠伤寒沙门氏菌umu测试菌株NM2009和NM3009中也呈阳性，这两种菌株分别高水平表达O-乙酰转移酶（O-AT）和O-AT加硝基还原酶（NR）。间硝基肉桂酸（m-NCA）在菌株NM2009和NM3009中呈弱阳性，但在SOS显色测试中呈阴性；间二硝基苯（m-DNB）在菌株NM2009中呈弱阳性，在菌株NM3009中呈中度阳性，但在SOS显色测试中呈阴性；2,4-二硝基甲苯（2,4-DNT）在菌株NM3009中呈弱阳性，但在菌株NM2009和SOS显色测试中呈阴性。然而，它在菌株NM2009中仍显示出剂量反应关系。鉴于这些结果，建议未来计划筛选各种硝基芳烃遗传毒性的研究人员考虑利用鼠伤寒沙门氏菌菌株NM2009和NM3009因过表达O-AT或O-AT和NR而具有的更高敏感性。
