一种生物测定法，用于测定植酸酶对肉鸡和蛋鸡饲料原料中植酸磷水解及总磷保留的影响。

A bioassay to determine the effect of phytase on phytate phosphorus hydrolysis and total phosphorus retention of feed ingredients as determined with broilers and laying hens.

作者信息

Leske K L, Coon C N

机构信息

Department of Poultry Science, University of Arkansas, Fayetteville 72701, USA.

出版信息

Poult Sci. 1999 Aug;78(8):1151-7. doi: 10.1093/ps/78.8.1151.

DOI:10.1093/ps/78.8.1151

PMID:10472841

Abstract

In order to accurately formulate diets for broilers and laying hens to meet phosphorus requirements without overfeeding, precise knowledge of an individual feed ingredient's contribution to the retainable phosphorus is needed. Seven feed ingredients, included as the sole source of phosphorus, were tested with and without the addition of 600 phytase units (FTU) phytase/kg diet, in a 5-d bioassay with 10 22-d-old male broilers. Without addition of phytase, the amounts of phytate phosphorus hydrolyzed in corn, soybean meal, wheat, wheat midds, barley, defatted rice bran, and canola were 30.8, 34.9, 30.7, 29.1, 32.2, 33.2, and 36.7%, respectively. The addition of phytase increased (P < or = 0.05) each value to 59.0, 72.4, 46.8, 52.2, 71.3, 48.0, and 55.8%, respectively. The addition of phytase increased total phosphorus retention from 34.8, 27.0, 16.0, 31.9, 40.3, 15.5, and 39.4% to 40.9, 58.0, 33.8, 43.4, 55.5, 26.5, and 45.7%, respectively. A similar bioassay was conducted with laying hens fed corn, soybean meal, and defatted rice bran. Without phytase addition, phytate phosphorus hydrolyzed in soybean meal, corn, and rice bran was determined to be 25.7, 23.0, and 36.1%, respectively, and was increased (P < or = 0.05) to 62.4, 52.0, and 50.9%, respectively, with the addition of 300 FTU phytase/kg feed. Total phosphorus retention of soybean meal, corn, and rice bran increased from 36.8, 28.6, and 35.9% to 53.4, 44.7, and 43.0%, respectively, with the addition of phytase.

摘要

为了准确制定肉鸡和蛋鸡的日粮，以满足磷需求且不过量饲喂，需要精确了解每种饲料原料对可留存磷的贡献。在一项为期5天的生物测定中，以10只22日龄雄性肉鸡为试验对象，对7种作为磷唯一来源的饲料原料进行了测试，每种原料分别在添加和不添加600植酸酶单位（FTU）/千克日粮的情况下进行。不添加植酸酶时，玉米、豆粕、小麦、小麦麸、大麦、脱脂米糠和油菜籽中植酸磷的水解量分别为30.8%、34.9%、30.7%、29.1%、32.2%、33.2%和36.7%。添加植酸酶后，各值分别增加（P≤0.05）至59.0%、72.4%、46.8%、52.2%、71.3%、48.0%和55.8%。添加植酸酶使总磷留存率分别从34.8%、27.0%、16.0%、31.9%、40.3%、15.5%和39.4%提高至40.9%、58.0%、33.8%、43.4%、55.5%、26.5%和45.7%。对饲喂玉米、豆粕和脱脂米糠的蛋鸡进行了类似的生物测定。不添加植酸酶时，豆粕、玉米和米糠中植酸磷的水解量分别测定为25.7%、23.0%和36.1%，添加300 FTU/千克饲料的植酸酶后，分别增加（P≤0.05）至62.4%、52.0%和50.9%。添加植酸酶后，豆粕、玉米和米糠的总磷留存率分别从36.8%、28.6%和35.9%提高至53.4%、44.7%和43.0%。