Almeida R A, Fang W, Oliver S P
Department of Animal Science, University of Tennessee, Knoxville 37996, USA.
FEMS Microbiol Lett. 1999 Aug 15;177(2):313-7. doi: 10.1111/j.1574-6968.1999.tb13748.x.
The role of cell glycosaminoglycans (GAG) on adherence and internalization of Streptococcus uberis to bovine mammary epithelial cells was evaluated by adherence/internalization competition assays, by removal of GAG from the host cell surface and by inhibition of GAG glycosylation in the host cell. Heparin (HEP), heparan sulfate (HSA), chondroitin sulfate A (CSA) and chondroitin sulfate B (CSB) inhibited adherence and internalization of S. uberis in a dose-dependent manner. However, inhibition was lower with CSA and CSB than that observed with HEP and HSA. Adherence and internalization were also inhibited upon treatment of mammary epithelial cells with GAG lyases. The greatest inhibition was observed with heparinase I. Tunicamycin, an inhibitor of mammalian cell glycosylation of cell surface glycoproteins, markedly inhibited internalization of S. uberis into mammary epithelial cells. Differences between strains were observed. These results suggest that a HSA proteoglycan receptor on the host cell surface may mediate S. uberis adherence to and internalization of bovine mammary epithelial cells.
通过黏附/内化竞争试验、从宿主细胞表面去除糖胺聚糖以及抑制宿主细胞中糖胺聚糖糖基化，评估了细胞糖胺聚糖（GAG）在乳房链球菌黏附及内化至牛乳腺上皮细胞过程中的作用。肝素（HEP）、硫酸乙酰肝素（HSA）、硫酸软骨素A（CSA）和硫酸软骨素B（CSB）以剂量依赖性方式抑制乳房链球菌的黏附和内化。然而，CSA和CSB的抑制作用低于HEP和HSA。用GAG裂解酶处理乳腺上皮细胞后，黏附和内化也受到抑制。肝素酶I的抑制作用最为明显。衣霉素是一种抑制哺乳动物细胞表面糖蛋白糖基化的抑制剂，它显著抑制乳房链球菌内化至乳腺上皮细胞。观察到不同菌株之间存在差异。这些结果表明，宿主细胞表面的硫酸乙酰肝素蛋白聚糖受体可能介导乳房链球菌黏附并内化至牛乳腺上皮细胞。
