一种38千道尔顿的核蛋白参与了针对胞质磷脂酶A2的反义寡核苷酸的滞留过程。
A 38 kDa nuclear protein is involved in the retention of an antisense oligonucleotide directed against cytosolic phospholipase A2.
作者信息
Griffoni C, Spisni E, Orlandi M, Santi S, Riccio M, Tomasi V
机构信息
Department of Experimental Biology, University of Bologna, Italy.
出版信息
Nucleosides Nucleotides. 1999 Jun-Jul;18(6-7):1673-6. doi: 10.1080/07328319908044819.
Recent studies suggest that antisense phosphorothioate oligonucleotides (APO) are useful tools not only to impair gene expression, but also to modify the splicing of pre-mRNA, as the classical view that they act by suppressing the translation of mature mRNA has been challenged by several examples showing their nuclear site of action. In this work we show that an APO directed against cytosolic phospholipase A2 (cPLA2) mRNA localises in the nucleus and interacts with a specific nuclear protein.
最近的研究表明，反义硫代磷酸酯寡核苷酸（APO）不仅是削弱基因表达的有用工具，而且还可用于修饰前体mRNA的剪接，因为它们通过抑制成熟mRNA的翻译而起作用这一经典观点已受到多个显示其核作用位点的例子的挑战。在这项工作中，我们表明，一种针对胞质磷脂酶A2（cPLA2）mRNA的APO定位于细胞核并与一种特定的核蛋白相互作用。
Zotero 插件