Karamysheva A F, Kobliakov V A, Mironov N M
Biull Eksp Biol Med. 1979 Jan;87(1):19-21.
Polycyclic aromatic hydrocarbons (PAH) were covalently bound to DNA by means of various activating systems. The following systems were used: the microsomal fraction of the rat liver, the system with I2, the system with ascorbic acid and FeSO4. Breaks in DNA due to the activating systems action appeared in all of these systems. Plateau of the PAH binding system curve in the microsomal system cannot be attributed either to the fall of the PAH metabolism rate to zero, or to the PAH binding sites in DNA. This plateau is the result of equalization of the rates of the two contrary-directed processes: the binding of metabolites and their removal due to DNA degradation. Because of the breaks in DNA caused by the activating systems, the authors failed to discover the changes in sedimentation data of DNA due to the covalently bound PAH.
多环芳烃（PAH）通过各种活化系统与DNA共价结合。使用了以下系统：大鼠肝脏微粒体部分、碘系统、抗坏血酸和硫酸亚铁系统。由于活化系统的作用，所有这些系统中都出现了DNA断裂。微粒体系统中PAH结合系统曲线的平稳期既不能归因于PAH代谢率降至零，也不能归因于DNA中的PAH结合位点。这个平稳期是两个相反方向过程的速率平衡的结果：代谢物的结合及其因DNA降解而被去除。由于活化系统导致DNA断裂，作者未能发现共价结合的PAH引起的DNA沉降数据的变化。
