Sestito A, Sciahbasi A, Landolfi R, Maseri A, Lanza G A, Andreotti F
Istituto di Cardiologia, Università Cattolica del Sacro Cuore, Roma.
Cardiologia. 1999 Jul;44(7):661-5.
A simple assay for platelet-mediated hemostasis in flowing blood (PFA-100) has become available. Whole blood is aspirated through the central opening of a membrane coated with platelet agonists; the time required for a platelet thrombus to occlude the opening is defined as closure time; the shorter the closure time the greater the platelet aggregability. There are limited data on the normal range of values for this test, and the effects of sex and age are not known. The aim of this study was to determine the effects of sex and age on closure time in normal volunteers and to assess the reproducibility of the test in our laboratory.
Closure time using collagen/adenosine-5'-diphosphate was measured in 62 apparently healthy individuals 35 to 75 years of age (11 men and 17 women < 55 years and 22 men and 12 women > 55 years).
Closure time was 96.6 +/- 24 s in men and 93.1 +/- 16 s in women (p = 0.20). In the entire group, closure time did not significantly correlate with age (r = -0.17, p = 0.18). However, men < 55 years tended to have a longer closure time than men > 55 years (109.7 +/- 23 vs 90.0 +/- 22 s, p = 0.08), whereas in women closure time was similar in those younger or older than 55 years (93.1 +/- 16 vs 93.3 +/- 18 s, respectively). In 20 samples tested in duplicate, the mean closure time was 80.9 +/- 10 s on the first determination and 81.5 +/- 12 s on the second (r = 0.89, p < 0.001). There was no significant correlation between closure time and hematocrit, platelet number, mean platelet volume, or leukocyte count.
The platelet function analyzer PFA-100 showed a good reproducibility in apparently healthy subjects. No significant difference in closure time was found between men and women, nor between subjects younger or older than 55 years, although a tendency towards shorter values was found in older compared with younger men.
一种用于检测流动血液中血小板介导的止血功能的简单检测方法（PFA - 100）已问世。全血通过涂有血小板激动剂的膜的中央开口被抽吸；血小板血栓阻塞开口所需的时间被定义为封闭时间；封闭时间越短，血小板聚集性越强。关于该检测正常参考值范围的数据有限，且性别和年龄对其的影响尚不清楚。本研究的目的是确定性别和年龄对正常志愿者封闭时间的影响，并评估该检测在我们实验室的可重复性。
对62名年龄在35至75岁的明显健康个体（年龄<55岁的男性11名、女性17名，年龄>55岁的男性22名、女性12名）测量使用胶原/腺苷 - 5'-二磷酸时的封闭时间。
男性的封闭时间为96.6±24秒，女性为93.1±16秒（p = 0.20）。在整个组中，封闭时间与年龄无显著相关性（r = -0.17，p = 0.18）。然而，年龄<55岁的男性的封闭时间往往比年龄>55岁的男性长（分别为109.7±23秒和90.0±22秒，p = 0.08），而在女性中，年龄<55岁和>55岁者的封闭时间相似（分别为93.1±16秒和93.3±18秒）。在20个进行重复检测的样本中，首次测定时平均封闭时间为80.9±10秒，第二次为81.5±12秒（r = 0.89，p<0.001）。封闭时间与血细胞比容、血小板计数、平均血小板体积或白细胞计数之间无显著相关性。
血小板功能分析仪PFA - 100在明显健康的受试者中显示出良好的可重复性。男性与女性之间、年龄<55岁和>55岁的受试者之间的封闭时间均未发现显著差异，尽管与年轻男性相比，老年男性的封闭时间有缩短的趋势。
